Multicenter evaluation of quantification methods for plasma human immunodeficiency virus type 1 rna

Hsiang Ju Lin; Lawrence E. Myers; Belinda Yen-Lieberman; F. Blaine Hollinger; Denis Henrard; Carol J. Hooper; Robert Kokka; Shirley Kwok; Suraiya Rasheed; Maryanne Vahey; Mark A. Winters; Lisa J. Mc Quay; Peter L. Nara; Patricia Reichelderfer; Robert W. Coombs; J. Brooks Jackson

doi:10.1093/infdis/170.3.553

Multicenter evaluation of quantification methods for plasma human immunodeficiency virus type 1 rna

Hsiang Ju Lin, Lawrence E. Myers, Belinda Yen-Lieberman, F. Blaine Hollinger, Denis Henrard, Carol J. Hooper, Robert Kokka, Shirley Kwok, Suraiya Rasheed, Maryanne Vahey, Mark A. Winters, Lisa J. Mc Quay, Peter L. Nara, Patricia Reichelderfer, Robert W. Coombs, J. Brooks Jackson

Research output: Contribution to journal › Article › peer-review

124 Scopus citations

Abstract

Six procedures for quantifying plasma human immunodeficiency virus type 1 (HIV-1) RNA were evaluated by nine laboratories. The procedures differed in their sample volume and preparation of samples and methods of amplification and detection. Coded samples in a IO-folddilution series of HIV-L-spiked plasma were correctly ranked by all six procedures. Subsequently, coded duplicate plasma samples from 16 HIV-I-infected patients were tested using a common set of standards. Several HIV-1 RNA procedures were sufficiently reproducible so that an empiric 4-fold change could be viewed as significant. HIV-I RNA levels in the patients (up to 370, 000 RNA copiesjmL) correlated with proviral HIV-I DNA and were inversely correlated with CD4 cell counts; HIV-1 RNA assays were more sensitive than plasma viremia, standard p24 antigen, or immune complex-dissociated p24 antigen assays. This study demonstrated that several HIV-1 RNA quantitative assays are ready for use in clinical trials.

Original language	English (US)
Pages (from-to)	553-562
Number of pages	10
Journal	Journal of Infectious Diseases
Volume	170
Issue number	3
DOIs	https://doi.org/10.1093/infdis/170.3.553
State	Published - 1994
Externally published	Yes

ASJC Scopus subject areas

Infectious Diseases
Immunology and Allergy
Immunology
Public Health, Environmental and Occupational Health

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10.1093/infdis/170.3.553

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Lin, H. J., Myers, L. E., Yen-Lieberman, B., Blaine Hollinger, F., Henrard, D., Hooper, C. J., Kokka, R., Kwok, S., Rasheed, S., Vahey, M., Winters, M. A., Mc Quay, L. J., Nara, P. L., Reichelderfer, P., Coombs, R. W., & Brooks Jackson, J. (1994). Multicenter evaluation of quantification methods for plasma human immunodeficiency virus type 1 rna. Journal of Infectious Diseases, 170(3), 553-562. https://doi.org/10.1093/infdis/170.3.553

Lin, HJ, Myers, LE, Yen-Lieberman, B, Blaine Hollinger, F, Henrard, D, Hooper, CJ, Kokka, R, Kwok, S, Rasheed, S, Vahey, M, Winters, MA, Mc Quay, LJ, Nara, PL, Reichelderfer, P, Coombs, RW & Brooks Jackson, J 1994, 'Multicenter evaluation of quantification methods for plasma human immunodeficiency virus type 1 rna', Journal of Infectious Diseases, vol. 170, no. 3, pp. 553-562. https://doi.org/10.1093/infdis/170.3.553

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abstract = "Six procedures for quantifying plasma human immunodeficiency virus type 1 (HIV-1) RNA were evaluated by nine laboratories. The procedures differed in their sample volume and preparation of samples and methods of amplification and detection. Coded samples in a IO-folddilution series of HIV-L-spiked plasma were correctly ranked by all six procedures. Subsequently, coded duplicate plasma samples from 16 HIV-I-infected patients were tested using a common set of standards. Several HIV-1 RNA procedures were sufficiently reproducible so that an empiric 4-fold change could be viewed as significant. HIV-I RNA levels in the patients (up to 370, 000 RNA copiesjmL) correlated with proviral HIV-I DNA and were inversely correlated with CD4 cell counts; HIV-1 RNA assays were more sensitive than plasma viremia, standard p24 antigen, or immune complex-dissociated p24 antigen assays. This study demonstrated that several HIV-1 RNA quantitative assays are ready for use in clinical trials.",

author = "Lin, {Hsiang Ju} and Myers, {Lawrence E.} and Belinda Yen-Lieberman and {Blaine Hollinger}, F. and Denis Henrard and Hooper, {Carol J.} and Robert Kokka and Shirley Kwok and Suraiya Rasheed and Maryanne Vahey and Winters, {Mark A.} and {Mc Quay}, {Lisa J.} and Nara, {Peter L.} and Patricia Reichelderfer and Coombs, {Robert W.} and {Brooks Jackson}, J.",

year = "1994",

doi = "10.1093/infdis/170.3.553",

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AU - Hooper, Carol J.

AU - Kokka, Robert

AU - Kwok, Shirley

AU - Rasheed, Suraiya

AU - Vahey, Maryanne

AU - Winters, Mark A.

AU - Mc Quay, Lisa J.

AU - Nara, Peter L.

AU - Reichelderfer, Patricia

AU - Coombs, Robert W.

AU - Brooks Jackson, J.

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Multicenter evaluation of quantification methods for plasma human immunodeficiency virus type 1 rna

Abstract

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