TY - JOUR
T1 - Multi-organ, multi-lineage engraftment by a single bone marrow-derived stem cell
AU - Krause, Diane S.
AU - Theise, Neil D.
AU - Collector, Michael I.
AU - Henegariu, Octavian
AU - Hwang, Sonya
AU - Gardner, Rebekah
AU - Neutzel, Sara
AU - Sharkis, Saul J.
N1 - Funding Information:
We thank Dr. Steven Baylin for critical reading of the manuscript, Dr. Richard Jones for helpful discussions, and Dr. Steven Piantadosi for statistical analysis. We thank Drs. Patricia Bray-Ward, David Ward, and Joseph Madri for generously allowing us to use their microscopes and image analysis software, Katherine Henderson for assistance with figure preparation, and Dr. Joanna Grove for advice and assistance. Supported by NIH grants: RO1 HL54330, RO1 DK53812, PO1 CA70970, RO1 DK53037, NIDDK P30-34989, PO1 HL63357, a Primary Biliary Cirrhosis Seed Grant from the American Liver Foundation, and funds from the Mary Lea Johnson Richards Research Institute and the Robert Krieger Memorial Fund.
PY - 2001/5/4
Y1 - 2001/5/4
N2 - Purification of rare hematopoietic stem cell(s) (HSC) to homogeneity is required to study their self-renewal, differentiation, phenotype, and homing. Long-term repopulation (LTR) of irradiated hosts and serial transplantation to secondary hosts represent the gold standard for demonstrating self-renewal and differentiation, the defining properties of HSC. We show that rare cells that home to bone marrow can LTR primary and secondary recipients. During the homing, CD34 and SCA-1 expression increases uniquely on cells that home to marrow. These adult bone marrow cells have tremendous differentiative capacity as they can also differentiate into epithelial cells of the liver, lung, GI tract, and skin. This finding may contribute to clinical treatment of genetic disease or tissue repair.
AB - Purification of rare hematopoietic stem cell(s) (HSC) to homogeneity is required to study their self-renewal, differentiation, phenotype, and homing. Long-term repopulation (LTR) of irradiated hosts and serial transplantation to secondary hosts represent the gold standard for demonstrating self-renewal and differentiation, the defining properties of HSC. We show that rare cells that home to bone marrow can LTR primary and secondary recipients. During the homing, CD34 and SCA-1 expression increases uniquely on cells that home to marrow. These adult bone marrow cells have tremendous differentiative capacity as they can also differentiate into epithelial cells of the liver, lung, GI tract, and skin. This finding may contribute to clinical treatment of genetic disease or tissue repair.
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U2 - 10.1016/S0092-8674(01)00328-2
DO - 10.1016/S0092-8674(01)00328-2
M3 - Article
C2 - 11348593
AN - SCOPUS:0035805055
SN - 0092-8674
VL - 105
SP - 369
EP - 377
JO - Cell
JF - Cell
IS - 3
ER -