Multi-omic profiles of hepatic metabolism in TPN-fed preterm pigs administered new generation lipid emulsions

Gregory Guthrie, Madhulika Kulkarni, Hester Vlaardingerbroek, Barbara Stoll, Kenneth Ng, Camilia Martin, John Belmont, Darryl Hadsell, William Heird, Christopher B. Newgard, Oluyinka Olutoye, Johannes Van Goudoever, Charlotte Lauridsen, Xingxuan He, Edward H. Schuchman, And Douglas Burrin

Research output: Contribution to journalArticle

Abstract

We aimed to characterize the lipidomic, metabolomic, and transcriptomic profiles in preterm piglets administered enteral (ENT) formula or three parenteral lipid emulsions [parenteral nutrition (PN)], Intralipid (IL), Omegaven (OV), or SMOFlipid (SL), for 14 days. Piglets in all parenteral lipid groups showed differential organ growth versus ENT piglets; whole body growth rate was lowest in IL piglets, yet there were no differences in either energy expenditure or13 C-palmitate oxidation. Plasma homeostatic model assessment of insulin resistance demonstrated insulin resistance in IL, but not OV or SL, compared with ENT. The fatty acid and acyl-CoA content of the liver, muscle, brain, and plasma fatty acids reflected the composition of the dietary lipids administered. Free carnitine and acylcarnitine (ACT) levels were markedly reduced in the PN groups compared with ENT piglets. Genes associated with oxidative stress and inflammation were increased, whereas those associated with alternative pathways of fatty acid oxidation were decreased in all PN groups. Our results show that new generation lipid emulsions directly enrich tissue fatty acids, especially in the brain, and lead to improved growth and insulin sensitivity compared with a soybean lipid emulsion. In all total PN groups, carnitine levels are limiting to the formation of ACTs and gene expression reflects the stress of excess lipid on liver function.-Guthrie, G., M. Kulkarni, H. Vlaardingerbroek, B. Stoll, K. Ng, C. Martin, J. Belmont, D. Hadsell, W. Heird, C. B. Newgard, O. Olutoye, J. van Goudoever, C. Lauridsen, X. He, E. H. Schuchman, and D. Burrin. Multi-omic profiles of hepatic metabolism in TPN-fed preterm pigs administered new generation lipid emulsions. J. Lipid Res.

Original languageEnglish (US)
Pages (from-to)1696-1711
Number of pages16
JournalJournal of Lipid Research
Volume57
Issue number9
DOIs
StatePublished - Sep 1 2016
Externally publishedYes

Fingerprint

Emulsions
Metabolism
Swine
Lipids
Liver
Nutrition
Small Intestine
Parenteral Nutrition
Fatty Acids
Insulin Resistance
Carnitine
Insulin
Brain
Growth
Plasmas
Oxidation
Acyl Coenzyme A
Oxidative stress
Metabolomics
Total Parenteral Nutrition

Keywords

  • Fish oil
  • Lipidomics
  • Medium-chain triglycerides
  • Metabolomics
  • Parenteral nutrition-associated liver disease
  • Soybean oil
  • Total parenteral nutrition
  • Transcriptomics
  • Vitamin E

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Cell Biology

Cite this

Guthrie, G., Kulkarni, M., Vlaardingerbroek, H., Stoll, B., Ng, K., Martin, C., ... Burrin, A. D. (2016). Multi-omic profiles of hepatic metabolism in TPN-fed preterm pigs administered new generation lipid emulsions. Journal of Lipid Research, 57(9), 1696-1711. https://doi.org/10.1194/jlr.M069526

Multi-omic profiles of hepatic metabolism in TPN-fed preterm pigs administered new generation lipid emulsions. / Guthrie, Gregory; Kulkarni, Madhulika; Vlaardingerbroek, Hester; Stoll, Barbara; Ng, Kenneth; Martin, Camilia; Belmont, John; Hadsell, Darryl; Heird, William; Newgard, Christopher B.; Olutoye, Oluyinka; Van Goudoever, Johannes; Lauridsen, Charlotte; He, Xingxuan; Schuchman, Edward H.; Burrin, And Douglas.

In: Journal of Lipid Research, Vol. 57, No. 9, 01.09.2016, p. 1696-1711.

Research output: Contribution to journalArticle

Guthrie, G, Kulkarni, M, Vlaardingerbroek, H, Stoll, B, Ng, K, Martin, C, Belmont, J, Hadsell, D, Heird, W, Newgard, CB, Olutoye, O, Van Goudoever, J, Lauridsen, C, He, X, Schuchman, EH & Burrin, AD 2016, 'Multi-omic profiles of hepatic metabolism in TPN-fed preterm pigs administered new generation lipid emulsions', Journal of Lipid Research, vol. 57, no. 9, pp. 1696-1711. https://doi.org/10.1194/jlr.M069526
Guthrie, Gregory ; Kulkarni, Madhulika ; Vlaardingerbroek, Hester ; Stoll, Barbara ; Ng, Kenneth ; Martin, Camilia ; Belmont, John ; Hadsell, Darryl ; Heird, William ; Newgard, Christopher B. ; Olutoye, Oluyinka ; Van Goudoever, Johannes ; Lauridsen, Charlotte ; He, Xingxuan ; Schuchman, Edward H. ; Burrin, And Douglas. / Multi-omic profiles of hepatic metabolism in TPN-fed preterm pigs administered new generation lipid emulsions. In: Journal of Lipid Research. 2016 ; Vol. 57, No. 9. pp. 1696-1711.
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AU - Stoll, Barbara

AU - Ng, Kenneth

AU - Martin, Camilia

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