MU switch region deletion is associated with both T cell independent and T cell dependent responses

Jean Mukherjee, Arturo Casadevall, Matthew D. Scharff

Research output: Contribution to journalArticle

Abstract

Isotype switching is a process by which immunoglobulin variable gene regions initially proximal to and expressed with the mu constant region gene (Cμ) can rearrange downstream to other constant region genes. Thus the same antigen binding site can be expressed with each of the other constant region isotypes and perform the full panoply of effector functions. Although isotype switching is thought to involve highly reiterated 'switch site' sequences located 5' to constant region genes, the exact role of these switch sites is unknown. It has been reported that prior to switching, the 'donor' switch site 5' of Cμ occasionally undergoes deletions, but it is not known whether this is an adventitious event or one which predisposes to or prevents isotype switching. Since T cell independent (TI) immune responses are dominated by IgM while T cell dependent (TD) responses are associated with switching to IgG, we have examined the state of the mu switch site in 51 IgM-producing hybridomas isolated from a variety of TI and TD responses. Although more hybridomas from the TI responses studied exhibited Sμ deletions, deletion of Sμ also occurred in hybridomas isolated from TD responses. Analysis of a well-characterized clonally related subset of IgM hybridomas also revealed that mu switch region deletion can be associated with the productive allele.

Original languageEnglish (US)
Pages (from-to)1049-1055
Number of pages7
JournalMolecular Immunology
Volume30
Issue number11
DOIs
StatePublished - 1993
Externally publishedYes

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Hybridomas
Immunoglobulin Class Switching
T-Lymphocytes
Immunoglobulin M
Immunoglobulin Variable Region
Genes
Immunoglobulin Genes
Immunoglobulin G
Alleles
Binding Sites
Antigens

ASJC Scopus subject areas

  • Molecular Biology
  • Immunology

Cite this

MU switch region deletion is associated with both T cell independent and T cell dependent responses. / Mukherjee, Jean; Casadevall, Arturo; Scharff, Matthew D.

In: Molecular Immunology, Vol. 30, No. 11, 1993, p. 1049-1055.

Research output: Contribution to journalArticle

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