TY - JOUR
T1 - MTORC1 inhibition is required for sensitivity to PI3K p110α inhibitors in PIK3CA-mutant breast cancer
AU - Elkabets, Moshe
AU - Vora, Sadhna
AU - Juric, Dejan
AU - Morse, Natasha
AU - Mino-Kenudson, Mari
AU - Muranen, Taru
AU - Tao, Jessica
AU - Campos, Ana Bosch
AU - Rodon, Jordi
AU - Ibrahim, Yasir H.
AU - Serra, Violeta
AU - Rodrik-Outmezguine, Vanessa
AU - Hazra, Saswati
AU - Singh, Sharat
AU - Kim, Phillip
AU - Quadt, Cornelia
AU - Liu, Manway
AU - Huang, Alan
AU - Rosen, Neal
AU - Engelman, Jeffrey A.
AU - Scaltriti, Maurizio
AU - Baselga, José
PY - 2013/7/31
Y1 - 2013/7/31
N2 - Activating mutations of the PIK3CA gene occur frequently in breast cancer, and inhibitors that are specific for phosphatidylinositol 3-kinase (PI3K) p110a, such as BYL719, are being investigated in clinical trials. In a search for correlates of sensitivity to p110a inhibition among PIK3CA-mutant breast cancer cell lines, we observed that sensitivity to BYL719 (as assessed by cell proliferation) was associated with full inhibition of signaling through the TORC1 pathway. Conversely, cancer cells that were resistant to BYL719 had persistently active mTORC1 signaling, although Akt phosphorylation was inhibited. Similarly, in patients, pS6 (residues 240/4) expression (a marker of mTORC1 signaling) was associated with tumor response to BYL719, and mTORC1 was found to be reactivated in tumors from patients whose disease progressed after treatment. In PIK3CA-mutant cancer cell lines with persistent mTORC1 signaling despite PI3K p110a blockade (that is, resistance), the addition of the allosteric mTORC1 inhibitor RAD001 to the cells alongwith BYL719 resulted in reversal of resistance in vitro and in vivo. Finally, we found that growth factors such as insulin-like growth factor 1 and neuregulin 1 can activatemammalian target of rapamycin (mTOR) and mediate resistance to BYL719. Our findings suggest that simultaneous administration of mTORC1 inhibitors may enhance the clinical activity of p110a-targeted drugs and delay the appearance of resistance.
AB - Activating mutations of the PIK3CA gene occur frequently in breast cancer, and inhibitors that are specific for phosphatidylinositol 3-kinase (PI3K) p110a, such as BYL719, are being investigated in clinical trials. In a search for correlates of sensitivity to p110a inhibition among PIK3CA-mutant breast cancer cell lines, we observed that sensitivity to BYL719 (as assessed by cell proliferation) was associated with full inhibition of signaling through the TORC1 pathway. Conversely, cancer cells that were resistant to BYL719 had persistently active mTORC1 signaling, although Akt phosphorylation was inhibited. Similarly, in patients, pS6 (residues 240/4) expression (a marker of mTORC1 signaling) was associated with tumor response to BYL719, and mTORC1 was found to be reactivated in tumors from patients whose disease progressed after treatment. In PIK3CA-mutant cancer cell lines with persistent mTORC1 signaling despite PI3K p110a blockade (that is, resistance), the addition of the allosteric mTORC1 inhibitor RAD001 to the cells alongwith BYL719 resulted in reversal of resistance in vitro and in vivo. Finally, we found that growth factors such as insulin-like growth factor 1 and neuregulin 1 can activatemammalian target of rapamycin (mTOR) and mediate resistance to BYL719. Our findings suggest that simultaneous administration of mTORC1 inhibitors may enhance the clinical activity of p110a-targeted drugs and delay the appearance of resistance.
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U2 - 10.1126/scitranslmed.3005747
DO - 10.1126/scitranslmed.3005747
M3 - Article
C2 - 23903756
AN - SCOPUS:84883624766
SN - 1946-6234
VL - 5
JO - Science translational medicine
JF - Science translational medicine
IS - 196
M1 - 196ra99
ER -