Mta has properties of an RNA export protein and increases cytoplasmic accumulation of Epstein-Barr virus replication gene mRNA

O. John Semmens, Lin Chen, Robert T. Sarisky, Zhigang Gao, Ling Zhong, S. Diane Hayward

Research output: Contribution to journalArticle

Abstract

The Epstein-Barr virus (EBV) Zta and Mta regulatory proteins were previously found to be required for efficient replication of oriLyt in cotransfection-replication assays, but the contribution of Mta to the replication process was unknown. We now demonstrate that Mta regulates replication gene expression. Using the polymerase processivity factor BMRF1 as an example, we found that in transfected cells, total BMRF1 mRNA levels were unaffected by Mta but that the amounts of cytoplasmic BMRF1 RNA and protein were greatly increased in the presence of Mta. Mta also increased cytoplasmic accumulation of the BALF2, BALF5, BSLF1, and BBLF4 replication gene mRNAs but did not affect cytoplasmic levels of BBLF2/3 mRNA. Thus, five of the six core replication genes require Mta for efficient accumulation of cytoplasmic RNA. The contribution of Mta to posttranscriptional RNA processing was examined. Examination of Mta localization in transfected cells by indirect immunofluorescence revealed that Mta colocalized with the splicing factor SC35. We also found that Mta has RNA binding activity. Glutathione S-transferase-Mta bound to BMRF1 and BMLF1 transcripts but not to a control cellular gene RNA. Mta contains a consensus leucine-rich nuclear export signal. Such signal sequences are characteristic of proteins that undergo nuclear export. Examination of Mta localization in a heterokaryon assay provided evidence that Mta shuttles between the nucleus and the cytoplasm. Our experiments indicate that Mta functions in RNA processing and transport and mediates cytoplasmic accumulation of a number of EBV early mRNAs.

Original languageEnglish (US)
Pages (from-to)9526-9534
Number of pages9
JournalJournal of Virology
Volume72
Issue number12
StatePublished - Dec 1998

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Human herpesvirus 4
Virus Replication
virus replication
Human Herpesvirus 4
RNA
Messenger RNA
Genes
Post Transcriptional RNA Processing
Proteins
genes
proteins
RNA Transport
Nuclear Export Signals
Cell Nucleus Active Transport
Indirect Fluorescent Antibody Technique
Protein Sorting Signals
Glutathione Transferase
Leucine
heterokaryon
Cytoplasm

ASJC Scopus subject areas

  • Immunology

Cite this

Semmens, O. J., Chen, L., Sarisky, R. T., Gao, Z., Zhong, L., & Hayward, S. D. (1998). Mta has properties of an RNA export protein and increases cytoplasmic accumulation of Epstein-Barr virus replication gene mRNA. Journal of Virology, 72(12), 9526-9534.

Mta has properties of an RNA export protein and increases cytoplasmic accumulation of Epstein-Barr virus replication gene mRNA. / Semmens, O. John; Chen, Lin; Sarisky, Robert T.; Gao, Zhigang; Zhong, Ling; Hayward, S. Diane.

In: Journal of Virology, Vol. 72, No. 12, 12.1998, p. 9526-9534.

Research output: Contribution to journalArticle

Semmens, OJ, Chen, L, Sarisky, RT, Gao, Z, Zhong, L & Hayward, SD 1998, 'Mta has properties of an RNA export protein and increases cytoplasmic accumulation of Epstein-Barr virus replication gene mRNA', Journal of Virology, vol. 72, no. 12, pp. 9526-9534.
Semmens, O. John ; Chen, Lin ; Sarisky, Robert T. ; Gao, Zhigang ; Zhong, Ling ; Hayward, S. Diane. / Mta has properties of an RNA export protein and increases cytoplasmic accumulation of Epstein-Barr virus replication gene mRNA. In: Journal of Virology. 1998 ; Vol. 72, No. 12. pp. 9526-9534.
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