MrpacC regulates sporulation, insect cuticle penetration and immune evasion in Metarhizium robertsii

Wei Huang, Yanfang Shang, Peilin Chen, Qiang Gao, Chengshu Wang

Research output: Contribution to journalArticle

Abstract

pH-responsive transcription factor of the PacC/Rim101 family governs adaptation to environment, development and virulence in many fungal pathogens. In this study, we report the functions of a PacC homologue, MrpacC, in an insect pathogenic fungus Metarhizium robertsii. The gene was highly transcribed in the fungus in alkaline conditions, and deletion of MrpacC impaired fungal responses to ambient pH and salt/metal challenges but not osmotic stress. We found that MrpacC is required for fungal full virulence by contributing to penetration of insect cuticles, mycosis of insect cadavers and evasion of host immunity. In MrpacC deletion strains, the chitinase but not protease activity was reduced, which was consistent with the downregulation of groups A and C chitinase genes. Further, the glucosyltransferase genes involved in cell wall remodelling and protein glycosylation were upregulated in ΔMrpacC. MrpacC transcriptional control of chitinase and glucosyltransferase genes was verified both by the presence of PacC consensus binding motif in gene promoter regions and the promoter DNA-binding assays. The results of this study not only advances the understanding of PacC function in fungal development and virulence, but will also facilitate future studies on the mechanism(s) behind the selective control of target genes by PacC.

Original languageEnglish (US)
Pages (from-to)994-1008
Number of pages15
JournalEnvironmental Microbiology
Volume17
Issue number4
DOIs
StatePublished - Apr 1 2015
Externally publishedYes

Fingerprint

Metarhizium
immune evasion
insect cuticle
Immune Evasion
sporulation
cuticle
Insects
penetration
insect
gene
chitinase
virulence
Genes
Glucosyltransferases
Virulence
Chitinases
glucosyltransferases
genes
Fungi
promoter regions

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

MrpacC regulates sporulation, insect cuticle penetration and immune evasion in Metarhizium robertsii. / Huang, Wei; Shang, Yanfang; Chen, Peilin; Gao, Qiang; Wang, Chengshu.

In: Environmental Microbiology, Vol. 17, No. 4, 01.04.2015, p. 994-1008.

Research output: Contribution to journalArticle

Huang, Wei ; Shang, Yanfang ; Chen, Peilin ; Gao, Qiang ; Wang, Chengshu. / MrpacC regulates sporulation, insect cuticle penetration and immune evasion in Metarhizium robertsii. In: Environmental Microbiology. 2015 ; Vol. 17, No. 4. pp. 994-1008.
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