mRNA for tissue-type plasminogen activator is present in lesional epidermis from patients with psoriasis, pemphigus, or bullous pemphigoid, but is not detected in normal epidermis

Janet Baird, Gerald Sylvan Lazarus, Dominique Belin, Jean Dominique Vassalli, Nathalie Busso, Pascale Gubler, Pamela J. Jensen

Research output: Contribution to journalArticle

Abstract

Plasminogen activator (PA), which catalyzes the conversion of plasminogen to the proteinase plasmin, has been implicated in a variety of cutaneous disorders. Lesional epidermis from patients with psoriasis, pemphigus, bullous pemphigoid, and Hailey-Hailey disease contains elevated levels of tissue-type PA (tPA) activity compared to non-lesional epidermis or to epidermis from normal individuals. In the present study, we have used Northern blot analysis to demonstrate that mRNA for tPA is detectable in lesions from patients with psoriasis, pemphigus, and bullous pemphigoid, but is not detectable in normal epidermis. These data strongly suggest that the tPA enzymatic activity present in lesional epidermis results from enhanced synthesis of the enzyme in situ, secondary to elevated steady-state levels of tPA mRNA. Cultured keratinocytes likewise are shown to contain tPA mRNA. Previous investigators have suggested that the phenotypes of keratinocytes in culture, psoriatic epidermis, and epidermis in the process of wound reepithelialization are comparable. Our findings, combined with those of other investigators, suggest that elevated tPA expression may be another common feature of epidermis under these circumstances.

Original languageEnglish (US)
Pages (from-to)548-552
Number of pages5
JournalJournal of Investigative Dermatology
Volume95
Issue number5
StatePublished - Nov 1990
Externally publishedYes

Fingerprint

Bullous Pemphigoid
Pemphigus
Tissue Plasminogen Activator
Psoriasis
Epidermis
Messenger RNA
Plasminogen Activators
Plasminogen
Fibrinolysin
Peptide Hydrolases
Keratinocytes
Benign Familial Pemphigus
Research Personnel
Enzymes
Northern Blotting
Phenotype
Skin
Wounds and Injuries

ASJC Scopus subject areas

  • Dermatology

Cite this

mRNA for tissue-type plasminogen activator is present in lesional epidermis from patients with psoriasis, pemphigus, or bullous pemphigoid, but is not detected in normal epidermis. / Baird, Janet; Lazarus, Gerald Sylvan; Belin, Dominique; Vassalli, Jean Dominique; Busso, Nathalie; Gubler, Pascale; Jensen, Pamela J.

In: Journal of Investigative Dermatology, Vol. 95, No. 5, 11.1990, p. 548-552.

Research output: Contribution to journalArticle

Baird, Janet ; Lazarus, Gerald Sylvan ; Belin, Dominique ; Vassalli, Jean Dominique ; Busso, Nathalie ; Gubler, Pascale ; Jensen, Pamela J. / mRNA for tissue-type plasminogen activator is present in lesional epidermis from patients with psoriasis, pemphigus, or bullous pemphigoid, but is not detected in normal epidermis. In: Journal of Investigative Dermatology. 1990 ; Vol. 95, No. 5. pp. 548-552.
@article{77a3293979cc42ceada914339da4b7c1,
title = "mRNA for tissue-type plasminogen activator is present in lesional epidermis from patients with psoriasis, pemphigus, or bullous pemphigoid, but is not detected in normal epidermis",
abstract = "Plasminogen activator (PA), which catalyzes the conversion of plasminogen to the proteinase plasmin, has been implicated in a variety of cutaneous disorders. Lesional epidermis from patients with psoriasis, pemphigus, bullous pemphigoid, and Hailey-Hailey disease contains elevated levels of tissue-type PA (tPA) activity compared to non-lesional epidermis or to epidermis from normal individuals. In the present study, we have used Northern blot analysis to demonstrate that mRNA for tPA is detectable in lesions from patients with psoriasis, pemphigus, and bullous pemphigoid, but is not detectable in normal epidermis. These data strongly suggest that the tPA enzymatic activity present in lesional epidermis results from enhanced synthesis of the enzyme in situ, secondary to elevated steady-state levels of tPA mRNA. Cultured keratinocytes likewise are shown to contain tPA mRNA. Previous investigators have suggested that the phenotypes of keratinocytes in culture, psoriatic epidermis, and epidermis in the process of wound reepithelialization are comparable. Our findings, combined with those of other investigators, suggest that elevated tPA expression may be another common feature of epidermis under these circumstances.",
author = "Janet Baird and Lazarus, {Gerald Sylvan} and Dominique Belin and Vassalli, {Jean Dominique} and Nathalie Busso and Pascale Gubler and Jensen, {Pamela J.}",
year = "1990",
month = "11",
language = "English (US)",
volume = "95",
pages = "548--552",
journal = "Journal of Investigative Dermatology",
issn = "0022-202X",
publisher = "Nature Publishing Group",
number = "5",

}

TY - JOUR

T1 - mRNA for tissue-type plasminogen activator is present in lesional epidermis from patients with psoriasis, pemphigus, or bullous pemphigoid, but is not detected in normal epidermis

AU - Baird, Janet

AU - Lazarus, Gerald Sylvan

AU - Belin, Dominique

AU - Vassalli, Jean Dominique

AU - Busso, Nathalie

AU - Gubler, Pascale

AU - Jensen, Pamela J.

PY - 1990/11

Y1 - 1990/11

N2 - Plasminogen activator (PA), which catalyzes the conversion of plasminogen to the proteinase plasmin, has been implicated in a variety of cutaneous disorders. Lesional epidermis from patients with psoriasis, pemphigus, bullous pemphigoid, and Hailey-Hailey disease contains elevated levels of tissue-type PA (tPA) activity compared to non-lesional epidermis or to epidermis from normal individuals. In the present study, we have used Northern blot analysis to demonstrate that mRNA for tPA is detectable in lesions from patients with psoriasis, pemphigus, and bullous pemphigoid, but is not detectable in normal epidermis. These data strongly suggest that the tPA enzymatic activity present in lesional epidermis results from enhanced synthesis of the enzyme in situ, secondary to elevated steady-state levels of tPA mRNA. Cultured keratinocytes likewise are shown to contain tPA mRNA. Previous investigators have suggested that the phenotypes of keratinocytes in culture, psoriatic epidermis, and epidermis in the process of wound reepithelialization are comparable. Our findings, combined with those of other investigators, suggest that elevated tPA expression may be another common feature of epidermis under these circumstances.

AB - Plasminogen activator (PA), which catalyzes the conversion of plasminogen to the proteinase plasmin, has been implicated in a variety of cutaneous disorders. Lesional epidermis from patients with psoriasis, pemphigus, bullous pemphigoid, and Hailey-Hailey disease contains elevated levels of tissue-type PA (tPA) activity compared to non-lesional epidermis or to epidermis from normal individuals. In the present study, we have used Northern blot analysis to demonstrate that mRNA for tPA is detectable in lesions from patients with psoriasis, pemphigus, and bullous pemphigoid, but is not detectable in normal epidermis. These data strongly suggest that the tPA enzymatic activity present in lesional epidermis results from enhanced synthesis of the enzyme in situ, secondary to elevated steady-state levels of tPA mRNA. Cultured keratinocytes likewise are shown to contain tPA mRNA. Previous investigators have suggested that the phenotypes of keratinocytes in culture, psoriatic epidermis, and epidermis in the process of wound reepithelialization are comparable. Our findings, combined with those of other investigators, suggest that elevated tPA expression may be another common feature of epidermis under these circumstances.

UR - http://www.scopus.com/inward/record.url?scp=0025254616&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025254616&partnerID=8YFLogxK

M3 - Article

C2 - 2121833

AN - SCOPUS:0025254616

VL - 95

SP - 548

EP - 552

JO - Journal of Investigative Dermatology

JF - Journal of Investigative Dermatology

SN - 0022-202X

IS - 5

ER -