MRI detection of bacterial brain abscesses and monitoring of antibiotic treatment using bacCEST

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Abstract

Purpose: To develop a new MRI method to detect and characterize brain abscesses using the CEST contrast inherently carried by bacterial cells, namely bacCEST. Methods: Bacteria S. aureus (ATCC #49775) and F98 and 9L glioma cells were injected stereotactically in the brains of F344 rats to form abscesses and tumors. The CEST signals of brain abscesses (n=4) and tumors (n=7) were acquired using 2 B1 values (i.e., 1 and 3μT) and compared. The bacCEST signal of the brain abscesses in the rats (n=3) receiving ampicillin (intraperitoneal injection 40mg/kg twice daily) was acquired before, 4 and 10 days after the treatment. Results: The bacCEST signal of S. aureus was characterized in vitro as a strong and broad signal in the range of 1 to 4ppm, with the maximum contrast occurring at 2.6ppm. The CEST signal in S. aureus-induced brain abscesses was significantly higher than that of contralateral parenchyma (p=.003). Moreover, thanks to their different B1 independence, brain abscesses and tumors could be effectively differentiated (p=.005) using ΔCEST(2.6ppm, 3μT-1μT), defined by the difference between the CEST signal (offset=2.6ppm) acquired using B1=3μT and that of 1μT. In treated rats, bacCEST MRI could detect the response of bacteria as early as 4 days after the antibiotic treatment (p=.035). Conclusion: BacCEST MRI provides a new imaging method to detect, discriminate, and monitor bacterial infection in deep-seated organs. Because no contrast agent is needed, such an approach has a great translational potential for detecting and monitoring bacterial infection in deep-seated organs.

Original languageEnglish (US)
JournalMagnetic Resonance in Medicine
DOIs
StateAccepted/In press - Jan 1 2018

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Brain Abscess
Anti-Bacterial Agents
Bacterial Infections
Bacteria
Therapeutics
Inbred F344 Rats
Ampicillin
Intraperitoneal Injections
Brain Neoplasms
Glioma
Abscess
Contrast Media
Neoplasms
Brain

Keywords

  • Bacterial infection
  • Brain abscess
  • CEST
  • MRI

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Cite this

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title = "MRI detection of bacterial brain abscesses and monitoring of antibiotic treatment using bacCEST",
abstract = "Purpose: To develop a new MRI method to detect and characterize brain abscesses using the CEST contrast inherently carried by bacterial cells, namely bacCEST. Methods: Bacteria S. aureus (ATCC #49775) and F98 and 9L glioma cells were injected stereotactically in the brains of F344 rats to form abscesses and tumors. The CEST signals of brain abscesses (n=4) and tumors (n=7) were acquired using 2 B1 values (i.e., 1 and 3μT) and compared. The bacCEST signal of the brain abscesses in the rats (n=3) receiving ampicillin (intraperitoneal injection 40mg/kg twice daily) was acquired before, 4 and 10 days after the treatment. Results: The bacCEST signal of S. aureus was characterized in vitro as a strong and broad signal in the range of 1 to 4ppm, with the maximum contrast occurring at 2.6ppm. The CEST signal in S. aureus-induced brain abscesses was significantly higher than that of contralateral parenchyma (p=.003). Moreover, thanks to their different B1 independence, brain abscesses and tumors could be effectively differentiated (p=.005) using ΔCEST(2.6ppm, 3μT-1μT), defined by the difference between the CEST signal (offset=2.6ppm) acquired using B1=3μT and that of 1μT. In treated rats, bacCEST MRI could detect the response of bacteria as early as 4 days after the antibiotic treatment (p=.035). Conclusion: BacCEST MRI provides a new imaging method to detect, discriminate, and monitor bacterial infection in deep-seated organs. Because no contrast agent is needed, such an approach has a great translational potential for detecting and monitoring bacterial infection in deep-seated organs.",
keywords = "Bacterial infection, Brain abscess, CEST, MRI",
author = "Jing Liu and Renyuan Bai and Yuguo Li and Verena Staedtke and Shuixing Zhang and {Van Zijl}, {Peter C} and Guanshu Liu",
year = "2018",
month = "1",
day = "1",
doi = "10.1002/mrm.27180",
language = "English (US)",
journal = "Magnetic Resonance in Medicine",
issn = "0740-3194",
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TY - JOUR

T1 - MRI detection of bacterial brain abscesses and monitoring of antibiotic treatment using bacCEST

AU - Liu, Jing

AU - Bai, Renyuan

AU - Li, Yuguo

AU - Staedtke, Verena

AU - Zhang, Shuixing

AU - Van Zijl, Peter C

AU - Liu, Guanshu

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Purpose: To develop a new MRI method to detect and characterize brain abscesses using the CEST contrast inherently carried by bacterial cells, namely bacCEST. Methods: Bacteria S. aureus (ATCC #49775) and F98 and 9L glioma cells were injected stereotactically in the brains of F344 rats to form abscesses and tumors. The CEST signals of brain abscesses (n=4) and tumors (n=7) were acquired using 2 B1 values (i.e., 1 and 3μT) and compared. The bacCEST signal of the brain abscesses in the rats (n=3) receiving ampicillin (intraperitoneal injection 40mg/kg twice daily) was acquired before, 4 and 10 days after the treatment. Results: The bacCEST signal of S. aureus was characterized in vitro as a strong and broad signal in the range of 1 to 4ppm, with the maximum contrast occurring at 2.6ppm. The CEST signal in S. aureus-induced brain abscesses was significantly higher than that of contralateral parenchyma (p=.003). Moreover, thanks to their different B1 independence, brain abscesses and tumors could be effectively differentiated (p=.005) using ΔCEST(2.6ppm, 3μT-1μT), defined by the difference between the CEST signal (offset=2.6ppm) acquired using B1=3μT and that of 1μT. In treated rats, bacCEST MRI could detect the response of bacteria as early as 4 days after the antibiotic treatment (p=.035). Conclusion: BacCEST MRI provides a new imaging method to detect, discriminate, and monitor bacterial infection in deep-seated organs. Because no contrast agent is needed, such an approach has a great translational potential for detecting and monitoring bacterial infection in deep-seated organs.

AB - Purpose: To develop a new MRI method to detect and characterize brain abscesses using the CEST contrast inherently carried by bacterial cells, namely bacCEST. Methods: Bacteria S. aureus (ATCC #49775) and F98 and 9L glioma cells were injected stereotactically in the brains of F344 rats to form abscesses and tumors. The CEST signals of brain abscesses (n=4) and tumors (n=7) were acquired using 2 B1 values (i.e., 1 and 3μT) and compared. The bacCEST signal of the brain abscesses in the rats (n=3) receiving ampicillin (intraperitoneal injection 40mg/kg twice daily) was acquired before, 4 and 10 days after the treatment. Results: The bacCEST signal of S. aureus was characterized in vitro as a strong and broad signal in the range of 1 to 4ppm, with the maximum contrast occurring at 2.6ppm. The CEST signal in S. aureus-induced brain abscesses was significantly higher than that of contralateral parenchyma (p=.003). Moreover, thanks to their different B1 independence, brain abscesses and tumors could be effectively differentiated (p=.005) using ΔCEST(2.6ppm, 3μT-1μT), defined by the difference between the CEST signal (offset=2.6ppm) acquired using B1=3μT and that of 1μT. In treated rats, bacCEST MRI could detect the response of bacteria as early as 4 days after the antibiotic treatment (p=.035). Conclusion: BacCEST MRI provides a new imaging method to detect, discriminate, and monitor bacterial infection in deep-seated organs. Because no contrast agent is needed, such an approach has a great translational potential for detecting and monitoring bacterial infection in deep-seated organs.

KW - Bacterial infection

KW - Brain abscess

KW - CEST

KW - MRI

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