MR tracking of transplanted cells with "positive contrast" using manganese oxide nanoparticles

Assaf A. Gilad, Piotr Walczak, Michael T Mcmahon, Bin Na Hyon, Hee Lee Jung, Kwangjin An, Taegwhan Hyeon, Peter C Van Zijl, Jeff W Bulte

Research output: Contribution to journalArticle


Rat glioma cells were labeled using electroporation with either manganese oxide (MnO) or superparamagnetic iron oxide (SPIO) nanoparticles. The viability and proliferation of SPIO-labeled cells (1.9 mg Fe/ml) or cells electroporated with a low dose of MnO (100 μg Mn/ml) was not significantly different from unlabeled cells; a higher MnO dose (785 μg Mn/ml) was found to be toxic. The cellular ion content was 0.1-0.3 pg Mn/cell and 4.4 pg Fe/cell, respectively, with cellular relaxivities of 2.5-4.8 s-1 (R1) and 45-84 s-1 (R2) for MnO-labeled cells. Labeled cells (SPIO and low-dose MnO) were each transplanted in contralateral brain hemispheres of rats and imaged in vivo at 9.4T. While SPIO-labeled cells produced a strong "negative contrast" due to the increase in R2, MnO-labeled cells produced "positive contrast" with an increased R1. Simultaneous imaging of both transplants with opposite contrast offers a method for MR "double labeling" of different cell populations.

Original languageEnglish (US)
Pages (from-to)1-7
Number of pages7
JournalMagnetic Resonance in Medicine
Issue number1
StatePublished - Jul 2008



  • Cellular imaging
  • Contrast agent
  • Iron oxide
  • Manganese oxide
  • Nanoparticles
  • Transplantation

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology

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