MR molecular imaging of the Her-2/neu receptor in breast cancer cells using targeted iron oxide nanoparticles

Research output: Contribution to journalArticlepeer-review

Abstract

MR molecular imaging is an exciting new frontier in the biomedical applications of MR. One of the clinically relevant targets is the tyrosine kinase Her-2/neu receptor, which has a significant role in staging and treating breast cancer. In this study Her-2/neu receptors were imaged in a panel of breast cancer cells expressing different numbers of the receptors on the cell membrane. Commercially available streptavidin-conjugated superparamagnetic nanoparticles were used as targeted MR contrast agent. The nanoparticles were directed to receptors prelabeled with a biotinylated monoclonal antibody and generated strong T2 MR contrast in Her-2/neu-expressing cells. The contrast observed in MR images was proportional to the expression level of Her-2/neu receptors determined independently with FACS analysis. In these experiments, iron oxide nanoparticles were attached to the cell surface and were not internalized into the cells, which is a major advantage for in vivo applications of the method.

Original languageEnglish (US)
Pages (from-to)403-408
Number of pages6
JournalMagnetic resonance in medicine
Volume49
Issue number3
DOIs
StatePublished - Mar 1 2003

Keywords

  • Avidin-biotin system
  • Her-2/neu receptors
  • Iron oxide nanoparticles
  • MRI

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

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