MR imaging of iron phagocytosis in intraluminal thrombi of abdominal aortic aneurysms in humans

Alain Nchimi, Olivier Defawe, Denis Brisbois, Thomas K Y Broussaud, Jean Olivier Defraigne, Paul Magotteaux, Brigitte Massart, Jean Michel Serfaty, Xavier Houard, Jean Baptiste Michel, Natzi Sakalihasan

Research output: Contribution to journalArticle

Abstract

Purpose: To prospectively determine if superparamagnetic iron oxide (SPIO)-enhanced magnetic resonance (MR) imaging could help visualize leukocyte phagocytic activities in human abdominal aortic aneurysms (AAAs). Materials and Methods: This study was approved by the institutional ethics committee; all patients gave informed consent. Preoperative MR imaging data, including unenhanced and SPIOenhanced T1-, T2*-, and T2-weighted transverse images of the entire AAA, obtained 1 hour after contrast enhancement from 15 patients (mean age, 72.7 years ± 8.2; range, 60-83 years), 10 men (mean age, 73.5 years ± 7.9; range, 60-83 years) and five women (mean age, 71.2 years ± 9.4; range 60-82), were retrospectively evaluated. Morphologic appearance and semiquantitative and contrast-to-noise ratio (CNR) analyses of the thrombi were performed. Thrombi were analyzed semiquantitatively at microscopy after staining with hematoxylin-eosin, CD68, and CD66b. Levels of promatrix metalloproteinase (pro-MMP)-2 and pro-MMP-9, MMP-2 and MMP-9, and their mRNA located in the thrombus were assessed by using zymography and quantitative reverse transcriptase polymerase chain reaction analysis. Nonparametric statistics of the Spearman rank correlation were calculated to evaluate correlations between the aneurysm thrombus signal level decrease after SPIO and the levels of CD68+, CD66b+ cells, pro-MMP-2 and pro-MMP-9, MMP-2 and MMP-9, and MMP-9 mRNA. Results: The pre-SPIO CNRs in the luminal sublayer of the thrombus and the deeper thrombus were - 10.20 6 12.69 and - 5.68 ± 10.38, respectively. After SPIO, the CNRs decreased to 2 21.34 6 13.07 ( P <.001) and - 12.44 6 14.56, respectively ( P , .012). There was a significant linear correlation between the thrombus signal level decrease and the levels of CD68+ and CD66b+ cells, pro-MMP-9, and MMP-9 mRNA ( P <.05). Conclusion: MR imaging allows in vivo demonstration of SPIO uptake at the luminal interface of the thrombus. This uptake is correlated to the abundance of leukocytes.

Original languageEnglish (US)
Pages (from-to)973-981
Number of pages9
JournalRadiology
Volume254
Issue number3
DOIs
StatePublished - Mar 2010
Externally publishedYes

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Abdominal Aortic Aneurysm
Phagocytosis
Thrombosis
Iron
Magnetic Resonance Imaging
Matrix Metalloproteinases
Matrix Metalloproteinase 2
Metalloproteases
Messenger RNA
Leukocytes
Ethics Committees
Matrix Metalloproteinase 9
Hematoxylin
Eosine Yellowish-(YS)
Nonparametric Statistics
Reverse Transcriptase Polymerase Chain Reaction
Informed Consent
Human Activities
Aneurysm
Noise

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Cite this

Nchimi, A., Defawe, O., Brisbois, D., Broussaud, T. K. Y., Defraigne, J. O., Magotteaux, P., ... Sakalihasan, N. (2010). MR imaging of iron phagocytosis in intraluminal thrombi of abdominal aortic aneurysms in humans. Radiology, 254(3), 973-981. https://doi.org/10.1148/radiol.09090657

MR imaging of iron phagocytosis in intraluminal thrombi of abdominal aortic aneurysms in humans. / Nchimi, Alain; Defawe, Olivier; Brisbois, Denis; Broussaud, Thomas K Y; Defraigne, Jean Olivier; Magotteaux, Paul; Massart, Brigitte; Serfaty, Jean Michel; Houard, Xavier; Michel, Jean Baptiste; Sakalihasan, Natzi.

In: Radiology, Vol. 254, No. 3, 03.2010, p. 973-981.

Research output: Contribution to journalArticle

Nchimi, A, Defawe, O, Brisbois, D, Broussaud, TKY, Defraigne, JO, Magotteaux, P, Massart, B, Serfaty, JM, Houard, X, Michel, JB & Sakalihasan, N 2010, 'MR imaging of iron phagocytosis in intraluminal thrombi of abdominal aortic aneurysms in humans', Radiology, vol. 254, no. 3, pp. 973-981. https://doi.org/10.1148/radiol.09090657
Nchimi A, Defawe O, Brisbois D, Broussaud TKY, Defraigne JO, Magotteaux P et al. MR imaging of iron phagocytosis in intraluminal thrombi of abdominal aortic aneurysms in humans. Radiology. 2010 Mar;254(3):973-981. https://doi.org/10.1148/radiol.09090657
Nchimi, Alain ; Defawe, Olivier ; Brisbois, Denis ; Broussaud, Thomas K Y ; Defraigne, Jean Olivier ; Magotteaux, Paul ; Massart, Brigitte ; Serfaty, Jean Michel ; Houard, Xavier ; Michel, Jean Baptiste ; Sakalihasan, Natzi. / MR imaging of iron phagocytosis in intraluminal thrombi of abdominal aortic aneurysms in humans. In: Radiology. 2010 ; Vol. 254, No. 3. pp. 973-981.
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abstract = "Purpose: To prospectively determine if superparamagnetic iron oxide (SPIO)-enhanced magnetic resonance (MR) imaging could help visualize leukocyte phagocytic activities in human abdominal aortic aneurysms (AAAs). Materials and Methods: This study was approved by the institutional ethics committee; all patients gave informed consent. Preoperative MR imaging data, including unenhanced and SPIOenhanced T1-, T2*-, and T2-weighted transverse images of the entire AAA, obtained 1 hour after contrast enhancement from 15 patients (mean age, 72.7 years ± 8.2; range, 60-83 years), 10 men (mean age, 73.5 years ± 7.9; range, 60-83 years) and five women (mean age, 71.2 years ± 9.4; range 60-82), were retrospectively evaluated. Morphologic appearance and semiquantitative and contrast-to-noise ratio (CNR) analyses of the thrombi were performed. Thrombi were analyzed semiquantitatively at microscopy after staining with hematoxylin-eosin, CD68, and CD66b. Levels of promatrix metalloproteinase (pro-MMP)-2 and pro-MMP-9, MMP-2 and MMP-9, and their mRNA located in the thrombus were assessed by using zymography and quantitative reverse transcriptase polymerase chain reaction analysis. Nonparametric statistics of the Spearman rank correlation were calculated to evaluate correlations between the aneurysm thrombus signal level decrease after SPIO and the levels of CD68+, CD66b+ cells, pro-MMP-2 and pro-MMP-9, MMP-2 and MMP-9, and MMP-9 mRNA. Results: The pre-SPIO CNRs in the luminal sublayer of the thrombus and the deeper thrombus were - 10.20 6 12.69 and - 5.68 ± 10.38, respectively. After SPIO, the CNRs decreased to 2 21.34 6 13.07 ( P <.001) and - 12.44 6 14.56, respectively ( P , .012). There was a significant linear correlation between the thrombus signal level decrease and the levels of CD68+ and CD66b+ cells, pro-MMP-9, and MMP-9 mRNA ( P <.05). Conclusion: MR imaging allows in vivo demonstration of SPIO uptake at the luminal interface of the thrombus. This uptake is correlated to the abundance of leukocytes.",
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T1 - MR imaging of iron phagocytosis in intraluminal thrombi of abdominal aortic aneurysms in humans

AU - Nchimi, Alain

AU - Defawe, Olivier

AU - Brisbois, Denis

AU - Broussaud, Thomas K Y

AU - Defraigne, Jean Olivier

AU - Magotteaux, Paul

AU - Massart, Brigitte

AU - Serfaty, Jean Michel

AU - Houard, Xavier

AU - Michel, Jean Baptiste

AU - Sakalihasan, Natzi

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N2 - Purpose: To prospectively determine if superparamagnetic iron oxide (SPIO)-enhanced magnetic resonance (MR) imaging could help visualize leukocyte phagocytic activities in human abdominal aortic aneurysms (AAAs). Materials and Methods: This study was approved by the institutional ethics committee; all patients gave informed consent. Preoperative MR imaging data, including unenhanced and SPIOenhanced T1-, T2*-, and T2-weighted transverse images of the entire AAA, obtained 1 hour after contrast enhancement from 15 patients (mean age, 72.7 years ± 8.2; range, 60-83 years), 10 men (mean age, 73.5 years ± 7.9; range, 60-83 years) and five women (mean age, 71.2 years ± 9.4; range 60-82), were retrospectively evaluated. Morphologic appearance and semiquantitative and contrast-to-noise ratio (CNR) analyses of the thrombi were performed. Thrombi were analyzed semiquantitatively at microscopy after staining with hematoxylin-eosin, CD68, and CD66b. Levels of promatrix metalloproteinase (pro-MMP)-2 and pro-MMP-9, MMP-2 and MMP-9, and their mRNA located in the thrombus were assessed by using zymography and quantitative reverse transcriptase polymerase chain reaction analysis. Nonparametric statistics of the Spearman rank correlation were calculated to evaluate correlations between the aneurysm thrombus signal level decrease after SPIO and the levels of CD68+, CD66b+ cells, pro-MMP-2 and pro-MMP-9, MMP-2 and MMP-9, and MMP-9 mRNA. Results: The pre-SPIO CNRs in the luminal sublayer of the thrombus and the deeper thrombus were - 10.20 6 12.69 and - 5.68 ± 10.38, respectively. After SPIO, the CNRs decreased to 2 21.34 6 13.07 ( P <.001) and - 12.44 6 14.56, respectively ( P , .012). There was a significant linear correlation between the thrombus signal level decrease and the levels of CD68+ and CD66b+ cells, pro-MMP-9, and MMP-9 mRNA ( P <.05). Conclusion: MR imaging allows in vivo demonstration of SPIO uptake at the luminal interface of the thrombus. This uptake is correlated to the abundance of leukocytes.

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