Abstract
The reliability of the transmembrane (TM) sequence assignments for membrane proteins (MPs) in standard sequence databases is uncertain because the vast majority are based on hydropathy plots. A database of MPs with dependable assignments is necessary for developing new computational tools for the prediction of MP structure. We have therefore created MPtopo, a database of MPs whose topologies have been verified experimentally by means of crystallography, gene fusion, and other methods. Tests using MPtopo strongly validated four existing MP topology-prediction algorithms. MPtopo is freely available over the internet and can be queried by means of an SQL-based search engine.
Original language | English (US) |
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Pages (from-to) | 455-458 |
Number of pages | 4 |
Journal | Protein Science |
Volume | 10 |
Issue number | 2 |
DOIs | |
State | Published - 2001 |
Externally published | Yes |
Keywords
- Hydropathy plots
- Prediction accuracy
- Protein structure prediction
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology