Mouse models of acute lung injury and ARDS

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The acute respiratory distress syndrome (ARDS) is a devastating illness characterized by severe hypoxemia and diffuse alveolar damage. Direct lung infection is the leading cause of ARDS and can be modeled in mice using sterile models of inflammation or live pathogens. In this chapter, two mouse models for ARDS are defined. These include an infectious model of ARDS driven by direct administration of Streptococcus pneumoniae and a sterile inflammatory model mediated by intratracheal administration of lipopolysaccharide. Methods for growth and preparation of Streptococcus pneumoniae are provided as methods to assess lung inflammation and injury.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages341-350
Number of pages10
DOIs
Publication statusPublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1809
ISSN (Print)1064-3745

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Keywords

  • Acute lung injury
  • ARDS

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

D'Alessio, F. (2018). Mouse models of acute lung injury and ARDS. In Methods in Molecular Biology (pp. 341-350). (Methods in Molecular Biology; Vol. 1809). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-8570-8_22