Mouse mast cells express the tryptic protease neuropsin/Prss19

Guang William Wong, Yi Yang, Shinsuke Yasuda, Lixin Li, Richard L. Stevens

Research output: Contribution to journalArticle

Abstract

The only tryptic enzymes identified so far in mouse mast cells (MCs) are three members of the chromosome 17A3.3 family of neutral proteases. Sequence analysis of a cDNA library revealed that BALB/c mouse bone marrow-derived MCs express neuropsin, a member of the chromosome 7B2 family of tryptic kallikreins. Kinetic studies revealed that neuropsin is expressed relatively early in MC development. As assessed immunohistochemically, the MCs residing in numerous connective tissues store neuropsin in their secretory granules. The finding that the neuropsin transcript is maximally expressed in the intestine at the height of a helminth infection indicates that MC-committed progenitors selectively increase their expression of neuropsin as they develop into mature mucosal MCs. This is the first report documenting the expression of neuropsin in an immune cell. Thus, it is now apparent that mouse MCs store at least two distinct families of tryptic-like proteases in their secretory granules.

Original languageEnglish (US)
Pages (from-to)320-325
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume303
Issue number1
DOIs
StatePublished - Mar 28 2003
Externally publishedYes

Fingerprint

Chromosomes
Mast Cells
Peptide Hydrolases
Kallikreins
Gene Library
Bone
Tissue
Secretory Vesicles
Kinetics
Enzymes
Helminths
Connective Tissue
Intestines
Sequence Analysis
Bone Marrow
Infection

Keywords

  • Kallikreins
  • Mast cells
  • Neuropsin
  • Prss19
  • Serine proteases
  • Tryptases

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Mouse mast cells express the tryptic protease neuropsin/Prss19. / Wong, Guang William; Yang, Yi; Yasuda, Shinsuke; Li, Lixin; Stevens, Richard L.

In: Biochemical and Biophysical Research Communications, Vol. 303, No. 1, 28.03.2003, p. 320-325.

Research output: Contribution to journalArticle

Wong, Guang William ; Yang, Yi ; Yasuda, Shinsuke ; Li, Lixin ; Stevens, Richard L. / Mouse mast cells express the tryptic protease neuropsin/Prss19. In: Biochemical and Biophysical Research Communications. 2003 ; Vol. 303, No. 1. pp. 320-325.
@article{37b798bd149249c88e63b947c406324a,
title = "Mouse mast cells express the tryptic protease neuropsin/Prss19",
abstract = "The only tryptic enzymes identified so far in mouse mast cells (MCs) are three members of the chromosome 17A3.3 family of neutral proteases. Sequence analysis of a cDNA library revealed that BALB/c mouse bone marrow-derived MCs express neuropsin, a member of the chromosome 7B2 family of tryptic kallikreins. Kinetic studies revealed that neuropsin is expressed relatively early in MC development. As assessed immunohistochemically, the MCs residing in numerous connective tissues store neuropsin in their secretory granules. The finding that the neuropsin transcript is maximally expressed in the intestine at the height of a helminth infection indicates that MC-committed progenitors selectively increase their expression of neuropsin as they develop into mature mucosal MCs. This is the first report documenting the expression of neuropsin in an immune cell. Thus, it is now apparent that mouse MCs store at least two distinct families of tryptic-like proteases in their secretory granules.",
keywords = "Kallikreins, Mast cells, Neuropsin, Prss19, Serine proteases, Tryptases",
author = "Wong, {Guang William} and Yi Yang and Shinsuke Yasuda and Lixin Li and Stevens, {Richard L.}",
year = "2003",
month = "3",
day = "28",
doi = "10.1016/S0006-291X(03)00339-5",
language = "English (US)",
volume = "303",
pages = "320--325",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Mouse mast cells express the tryptic protease neuropsin/Prss19

AU - Wong, Guang William

AU - Yang, Yi

AU - Yasuda, Shinsuke

AU - Li, Lixin

AU - Stevens, Richard L.

PY - 2003/3/28

Y1 - 2003/3/28

N2 - The only tryptic enzymes identified so far in mouse mast cells (MCs) are three members of the chromosome 17A3.3 family of neutral proteases. Sequence analysis of a cDNA library revealed that BALB/c mouse bone marrow-derived MCs express neuropsin, a member of the chromosome 7B2 family of tryptic kallikreins. Kinetic studies revealed that neuropsin is expressed relatively early in MC development. As assessed immunohistochemically, the MCs residing in numerous connective tissues store neuropsin in their secretory granules. The finding that the neuropsin transcript is maximally expressed in the intestine at the height of a helminth infection indicates that MC-committed progenitors selectively increase their expression of neuropsin as they develop into mature mucosal MCs. This is the first report documenting the expression of neuropsin in an immune cell. Thus, it is now apparent that mouse MCs store at least two distinct families of tryptic-like proteases in their secretory granules.

AB - The only tryptic enzymes identified so far in mouse mast cells (MCs) are three members of the chromosome 17A3.3 family of neutral proteases. Sequence analysis of a cDNA library revealed that BALB/c mouse bone marrow-derived MCs express neuropsin, a member of the chromosome 7B2 family of tryptic kallikreins. Kinetic studies revealed that neuropsin is expressed relatively early in MC development. As assessed immunohistochemically, the MCs residing in numerous connective tissues store neuropsin in their secretory granules. The finding that the neuropsin transcript is maximally expressed in the intestine at the height of a helminth infection indicates that MC-committed progenitors selectively increase their expression of neuropsin as they develop into mature mucosal MCs. This is the first report documenting the expression of neuropsin in an immune cell. Thus, it is now apparent that mouse MCs store at least two distinct families of tryptic-like proteases in their secretory granules.

KW - Kallikreins

KW - Mast cells

KW - Neuropsin

KW - Prss19

KW - Serine proteases

KW - Tryptases

UR - http://www.scopus.com/inward/record.url?scp=0037470780&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037470780&partnerID=8YFLogxK

U2 - 10.1016/S0006-291X(03)00339-5

DO - 10.1016/S0006-291X(03)00339-5

M3 - Article

VL - 303

SP - 320

EP - 325

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 1

ER -