Mouse hind limb transplantation: A new composite tissue allotransplantation model using nonsuture supermicrosurgery

Robert Sucher, Cheng Hung Lin, Rami Zanoun, Komli Kofi Atsina, Matthias Weinstock, Benson Pulikkotil, Stefan Schneeberger, Xin Xiao Zheng, Johann Pratschke, W P Andrew Lee, Gerald Brandacher

Research output: Contribution to journalArticle

Abstract

Background. The development of microsurgical techniques has facilitated the establishment of vascularized composite tissue transplant models in small mammals. Because the mouse would be the ideal model to study various composite tissue allotransplantation (CTA)-related problems, we designed two new surgical techniques for orthotopic (ORT) and heterotopic (HET) hind limb transplantation. Methods. BALB/c hind limbs were transplanted to BALB/c or C57BL6 recipients using a nonsuture cuff technique. ORT: donor femoral vessels were anastomosed to recipient femoral vessels, the sciatic nerve approximated end-to-end and osteosynthesis was performed using an intramedullary rod. HET/cervical: Donor femoral vessels of a reduced size osteomyocutaneous hind limb CTA were anastomosed to recipient common carotid artery and external jugular vein without nerve approximation. Results. Both procedures could be performed with a high success rate (ORT: 62%; HET: 90%). Donor operation lasted for 100±12 min and recipient operation 114±27 min (ORT) and 54±16 min (HET). Complication rates in terms of bleeding, and thrombosis at the cuff side was slightly higher in the ORT group. All syngeneic grafts survived long term (>100 days). FK506 (2 mg/kg) significantly prolonged graft survival (87±22 days) when compared with untreated controls (6±1 day). Functional evaluation of ORT grafts by means of video gait kinematics and CatWalk analysis revealed specific differences of gait parameters when compared with nontransplanted controls (P

Original languageEnglish (US)
Pages (from-to)1374-1380
Number of pages7
JournalTransplantation
Volume90
Issue number12
DOIs
StatePublished - Dec 27 2010
Externally publishedYes

Fingerprint

Vascularized Composite Allotransplantation
Thigh
Extremities
Transplantation
Transplants
Gait
Common Carotid Artery
Jugular Veins
Tacrolimus
Sciatic Nerve
Graft Survival
Biomechanical Phenomena
Mammals
Thrombosis
Hemorrhage

Keywords

  • CatWalk
  • Composite tissue
  • Cuff technique
  • Mouse
  • Video gait kinematics

ASJC Scopus subject areas

  • Transplantation

Cite this

Mouse hind limb transplantation : A new composite tissue allotransplantation model using nonsuture supermicrosurgery. / Sucher, Robert; Lin, Cheng Hung; Zanoun, Rami; Atsina, Komli Kofi; Weinstock, Matthias; Pulikkotil, Benson; Schneeberger, Stefan; Zheng, Xin Xiao; Pratschke, Johann; Lee, W P Andrew; Brandacher, Gerald.

In: Transplantation, Vol. 90, No. 12, 27.12.2010, p. 1374-1380.

Research output: Contribution to journalArticle

Sucher, R, Lin, CH, Zanoun, R, Atsina, KK, Weinstock, M, Pulikkotil, B, Schneeberger, S, Zheng, XX, Pratschke, J, Lee, WPA & Brandacher, G 2010, 'Mouse hind limb transplantation: A new composite tissue allotransplantation model using nonsuture supermicrosurgery', Transplantation, vol. 90, no. 12, pp. 1374-1380. https://doi.org/10.1097/TP.0b013e3181ff4fc3
Sucher, Robert ; Lin, Cheng Hung ; Zanoun, Rami ; Atsina, Komli Kofi ; Weinstock, Matthias ; Pulikkotil, Benson ; Schneeberger, Stefan ; Zheng, Xin Xiao ; Pratschke, Johann ; Lee, W P Andrew ; Brandacher, Gerald. / Mouse hind limb transplantation : A new composite tissue allotransplantation model using nonsuture supermicrosurgery. In: Transplantation. 2010 ; Vol. 90, No. 12. pp. 1374-1380.
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AU - Schneeberger, Stefan

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