Motility and segregation of Hsp104-associated protein aggregates in budding yeast

Chuankai Zhou, Brian D. Slaughter, Jay R. Unruh, Amr Eldakak, Boris Rubinstein, Rong Li

Research output: Contribution to journalArticle

Abstract

During yeast cell division, aggregates of damaged proteins are segregated asymmetrically between the bud and the mother. It is thought that protein aggregates are cleared from the bud via actin cable-based retrograde transport toward the mother and that Bni1p formin regulates this transport. Here, we examined the dynamics of Hsp104-associated protein aggregates by video microscopy, particle tracking, and image correlation analysis. We show that protein aggregates undergo random walk without directional bias. Clearance of heat-induced aggregates from the bud does not depend on formin proteins but occurs mostly through dissolution via Hsp104p chaperon. Aggregates formed naturally in aged cells also exhibit random walk but do not dissolve during observation. Although our data do not disagree with a role for actin or cell polarity in aggregate segregation, modeling suggests that their asymmetric inheritance can be a predictable outcome of aggregates' slow diffusion and the geometry of yeast cells.

Original languageEnglish (US)
Pages (from-to)1186-1196
Number of pages11
JournalCell
Volume147
Issue number5
DOIs
StatePublished - Nov 23 2011
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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    Zhou, C., Slaughter, B. D., Unruh, J. R., Eldakak, A., Rubinstein, B., & Li, R. (2011). Motility and segregation of Hsp104-associated protein aggregates in budding yeast. Cell, 147(5), 1186-1196. https://doi.org/10.1016/j.cell.2011.11.002