Mosquito heparan sulfate and its potential role in malaria infection and transmission

Photini Sinnis, Alida Coppi, Toshihiko Toida, Hidenao Toyoda, Akiko Kinoshita-Toyoda, Jin Xie, Melissa M. Kemp, Robert J. Linhardt

Research output: Contribution to journalArticle

Abstract

Heparan sulfate has been isolated for the first time from the mosquito Anopheles stephensi, a known vector for Plasmodium parasites, the causative agents of malaria. Chondroitin sulfate, but not dermatan sulfate or hyaluronan, was also present in the mosquito. The glycosaminoglycans were isolated, from salivary glands and midguts of the mosquito in quantities sufficient for disaccharide microanalysis. Both of these organs are invaded at different stages of the Plasmodium life cycle. Mosquito heparan sulfate was found to contain the critical trisulfated disaccharide sequence, →4)β-D-GlcNS6S(1 → 4)-α-L-IdoA2S(1→, that is commonly found in human liver heparan sulfate, which serves as the receptor for apolipoprotein E and is also believed to be responsible for binding to the circumsporozoite protein found on the surface of the Plasmodium sporozoite. The heparan sulfate isolated from the whole mosquito binds to circumsporozoite protein, suggesting a role within the mosquito for infection and transmission of the Plasmodium parasite.

Original languageEnglish (US)
Pages (from-to)25376-25384
Number of pages9
JournalJournal of Biological Chemistry
Volume282
Issue number35
DOIs
StatePublished - Aug 31 2007
Externally publishedYes

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Infectious Disease Transmission
Heparitin Sulfate
Culicidae
Malaria
Plasmodium
Disaccharides
Low Density Lipoprotein Receptor-Related Protein-1
Dermatan Sulfate
Chondroitin Sulfates
Parasites
Hyaluronic Acid
Glycosaminoglycans
Liver
Life cycle
Sporozoites
Proteins
Anopheles
Salivary Glands
Life Cycle Stages

ASJC Scopus subject areas

  • Biochemistry

Cite this

Sinnis, P., Coppi, A., Toida, T., Toyoda, H., Kinoshita-Toyoda, A., Xie, J., ... Linhardt, R. J. (2007). Mosquito heparan sulfate and its potential role in malaria infection and transmission. Journal of Biological Chemistry, 282(35), 25376-25384. https://doi.org/10.1074/jbc.M704698200

Mosquito heparan sulfate and its potential role in malaria infection and transmission. / Sinnis, Photini; Coppi, Alida; Toida, Toshihiko; Toyoda, Hidenao; Kinoshita-Toyoda, Akiko; Xie, Jin; Kemp, Melissa M.; Linhardt, Robert J.

In: Journal of Biological Chemistry, Vol. 282, No. 35, 31.08.2007, p. 25376-25384.

Research output: Contribution to journalArticle

Sinnis, P, Coppi, A, Toida, T, Toyoda, H, Kinoshita-Toyoda, A, Xie, J, Kemp, MM & Linhardt, RJ 2007, 'Mosquito heparan sulfate and its potential role in malaria infection and transmission', Journal of Biological Chemistry, vol. 282, no. 35, pp. 25376-25384. https://doi.org/10.1074/jbc.M704698200
Sinnis, Photini ; Coppi, Alida ; Toida, Toshihiko ; Toyoda, Hidenao ; Kinoshita-Toyoda, Akiko ; Xie, Jin ; Kemp, Melissa M. ; Linhardt, Robert J. / Mosquito heparan sulfate and its potential role in malaria infection and transmission. In: Journal of Biological Chemistry. 2007 ; Vol. 282, No. 35. pp. 25376-25384.
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