Mosaic allelic insulin-like growth factor 2 expression patterns reveal a link between Wilms' tumorigenesis and epigenetic heterogeneity

Rolf Ohlsson, Hengmi Cui, Liangmei He, Susan Pfeifer, Helena Malmikumpu, Shan Jiang, Andrew P. Feinberg, Fredrik Hedborg

Research output: Contribution to journalArticlepeer-review

Abstract

Numerous observations link the loss of imprinting of insulin-like growth factor 2 (IGF2) and an overdosage of this growth factor gene with cancer, in general, and with Wilms' tumorigenesis, in particular. It is not known, however, if loss of imprinting correlates with specific stages of neoplasia or if allelic expression patterns vary within the tumor. By applying an allele-specific in situ hybridization technique to formalin-fixed thin sections, we show that the parental IGF2 alleles can be differentially expressed, not only in Wilms' tumors, but also in nephrogenic rests (which represent premalignant lesions) of Wilms' tumor patients. Moreover, a subpopulation of mesenchymal cells, which surrounds tumor nodules, expresses IGF2 biallelically irrespective of the imprinted state of IGF2 within the tumor. These data show that Wilms' tumorigenesis involves epigenetic heterogeneity as visualized by variable allelic IGF2 expression patterns.

Original languageEnglish (US)
Pages (from-to)3889-3892
Number of pages4
JournalCancer Research
Volume59
Issue number16
StatePublished - Aug 15 1999

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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