TY - JOUR
T1 - Morphology of Pig Retinal Pigment Epithelium Maintained in Organ Culture
AU - Del Priore, Lucian V.
AU - Glaser, Bert M.
AU - Quigley, Harry A.
AU - Dorman, Mary Ellen
AU - Green, W. Richard
N1 - Copyright:
Copyright 2015 Elsevier B.V., All rights reserved.
PY - 1988/9
Y1 - 1988/9
N2 - Exoplants of porcine retinal pigment epithelium (RPE) attached to Bruch's membrane, choroid, and sclera were maintained in organ culture for up to four weeks. Four-millimeter round buttons of eye wall that contained RPE, choroid, and sclera were trephined from freshly enucleated pig eyes and incubated at 37°C in Eagle's minimum essential medium with 10% fetal calf serum. The RPE cells remained as a monolayer for at least four weeks in organ culture, and individual RPE cells became taller and dome shaped. The RPE cells retained several prominent ultrastructural features, including apical microvilli, intracellular melanosomes and mitochondria, and intercellular tight junctions. Since the cellular substratum can exert important influences on cell behavior, the ability to maintain RPE cells attached to Bruch's membrane provides a new in vitro tool for studying the metabolic activity of this tissue and its response to external stimuli, including laser photocoagulation.
AB - Exoplants of porcine retinal pigment epithelium (RPE) attached to Bruch's membrane, choroid, and sclera were maintained in organ culture for up to four weeks. Four-millimeter round buttons of eye wall that contained RPE, choroid, and sclera were trephined from freshly enucleated pig eyes and incubated at 37°C in Eagle's minimum essential medium with 10% fetal calf serum. The RPE cells remained as a monolayer for at least four weeks in organ culture, and individual RPE cells became taller and dome shaped. The RPE cells retained several prominent ultrastructural features, including apical microvilli, intracellular melanosomes and mitochondria, and intercellular tight junctions. Since the cellular substratum can exert important influences on cell behavior, the ability to maintain RPE cells attached to Bruch's membrane provides a new in vitro tool for studying the metabolic activity of this tissue and its response to external stimuli, including laser photocoagulation.
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U2 - 10.1001/archopht.1988.01060140446050
DO - 10.1001/archopht.1988.01060140446050
M3 - Article
C2 - 3415555
AN - SCOPUS:0023740894
SN - 0003-9950
VL - 106
SP - 1286
EP - 1290
JO - Archives of ophthalmology
JF - Archives of ophthalmology
IS - 9
ER -