Morphological and molecular identification of Spirocerca lupi (Nematoda: Spiruridae) found in the Andean fox (Lycalopex culpaeus)

Luis A. Gomez-Puerta, Johan Carrasco, Maria T. Lopez-Urbina, Armandoe Gonzalez

Research output: Contribution to journalArticle

Abstract

Lesions compatible with spirocercosis were found in the esophagus and aorta of an Andean fox from Cuzco, Peru. The esophageal and aortic lesions were 5.5 and 1.5 cm in diameter, respectively. A total of 12 adult nematodes (6 males and 6 females) were collected from the esophageal lesion, and all were identified as Spirocerca lupi by morphological and molecular methods. Molecular characterization was performed by analyzing two sources of the cox1 gene, and the sequences were compared with previous S. lupi sequences from other work deposited in GenBank. Analysis of the partial cox1 gene from S. lupi (n = 3) showed 2 haplotypes and had 95–99% nucleotide similarity to previously described sequences. Also, molecular analysis showed that S. lupi is a very diverse group, due to the genetic variability of the partial sequences of the cox1 gene of Spirocerca. This study is the first to report finding of spirocercosis in the Andean fox.

Original languageEnglish (US)
Pages (from-to)1-6
Number of pages6
JournalJournal of Parasitic Diseases
DOIs
StateAccepted/In press - May 24 2018
Externally publishedYes

Fingerprint

Thelazioidea
Nematoda
Genes
Peru
Nucleic Acid Databases
Haplotypes
Esophagus
Aorta
Nucleotides

Keywords

  • Andean fox
  • Culpeo fox
  • Cytochrome c oxidase 1 gene
  • Lycalopex culpaeus
  • Nematode
  • Spirocerca lupi
  • Spirocercosis
  • Spirurid

ASJC Scopus subject areas

  • Parasitology

Cite this

Morphological and molecular identification of Spirocerca lupi (Nematoda : Spiruridae) found in the Andean fox (Lycalopex culpaeus). / Gomez-Puerta, Luis A.; Carrasco, Johan; Lopez-Urbina, Maria T.; Gonzalez, Armandoe.

In: Journal of Parasitic Diseases, 24.05.2018, p. 1-6.

Research output: Contribution to journalArticle

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abstract = "Lesions compatible with spirocercosis were found in the esophagus and aorta of an Andean fox from Cuzco, Peru. The esophageal and aortic lesions were 5.5 and 1.5 cm in diameter, respectively. A total of 12 adult nematodes (6 males and 6 females) were collected from the esophageal lesion, and all were identified as Spirocerca lupi by morphological and molecular methods. Molecular characterization was performed by analyzing two sources of the cox1 gene, and the sequences were compared with previous S. lupi sequences from other work deposited in GenBank. Analysis of the partial cox1 gene from S. lupi (n = 3) showed 2 haplotypes and had 95–99{\%} nucleotide similarity to previously described sequences. Also, molecular analysis showed that S. lupi is a very diverse group, due to the genetic variability of the partial sequences of the cox1 gene of Spirocerca. This study is the first to report finding of spirocercosis in the Andean fox.",
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