TY - JOUR
T1 - Morphological and functional characteristics of aging kidneys based on two-photon microscopy in vivo
AU - Wu, Shulian
AU - Guo, Hengchang
AU - Horng, Hannah
AU - Liu, Yi
AU - Li, Hui
AU - Daneshpajouhnejad, Parnaz
AU - Rosenberg, Avi
AU - Albanese, Christopher
AU - Ranjit, Suman
AU - Andrews, Peter M.
AU - Levi, Moshe
AU - Tang, Qinggong
AU - Chen, Yu
N1 - Funding Information:
Yu Chen would like to acknowledge the National Institutes of Health (NIH) grant R21 AG042700. Shulian Wu would like to acknowledge the Special Funds of the Central Government Guiding Local Science and Technology Development (2017L3009).
Funding Information:
Yu Chen would like to acknowledge the National Institutes of Health (NIH) grant R21 AG042700. Shulian Wu would like to acknowledge the Special Funds of the Central Government Guiding Local Science and Technology Development (2017L3009).
Publisher Copyright:
© 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
PY - 2020/2/1
Y1 - 2020/2/1
N2 - Age-related kidney disease, which is chronic and naturally occurring, is a general term for a set of heterogeneous disorders affecting kidney structures and characterized by a decline in renal function. Age-related renal insufficiency has important implications with regard to body homeostasis, drug toxicity and renal transplantation. In our study, two-photon microscopy was used to image kidney morphological and functional characteristics in an age-related rat model in vivo. The changes in morphology are analyzed based on autofluorescence and Hoechst 33342 labeling in rats with different ages. Structural parameters including renal tubular diameter, cell nuclei density, size and shape are studied and compared with Hematoxylin and Eosin histological analysis. Functional characteristics, such as blood flow, and glomerular filtration rate are studied with high-molecular weight (MW) 500-kDa dextran-fluorescein and low-MW 10-kDa dextran-rhodamine. Results indicate that morphology changes significantly and functional characteristics deteriorate with age. These parameters are potential indicators for evaluating age-related renal morphology and function changes. Combined analyses of these parameters could provide a quantitative, novel method for monitoring kidney diseases and/or therapeutic effects of kidney drugs.
AB - Age-related kidney disease, which is chronic and naturally occurring, is a general term for a set of heterogeneous disorders affecting kidney structures and characterized by a decline in renal function. Age-related renal insufficiency has important implications with regard to body homeostasis, drug toxicity and renal transplantation. In our study, two-photon microscopy was used to image kidney morphological and functional characteristics in an age-related rat model in vivo. The changes in morphology are analyzed based on autofluorescence and Hoechst 33342 labeling in rats with different ages. Structural parameters including renal tubular diameter, cell nuclei density, size and shape are studied and compared with Hematoxylin and Eosin histological analysis. Functional characteristics, such as blood flow, and glomerular filtration rate are studied with high-molecular weight (MW) 500-kDa dextran-fluorescein and low-MW 10-kDa dextran-rhodamine. Results indicate that morphology changes significantly and functional characteristics deteriorate with age. These parameters are potential indicators for evaluating age-related renal morphology and function changes. Combined analyses of these parameters could provide a quantitative, novel method for monitoring kidney diseases and/or therapeutic effects of kidney drugs.
KW - age-related kidney
KW - renal function
KW - renal morphology
KW - two-photon microscopy
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U2 - 10.1002/jbio.201900246
DO - 10.1002/jbio.201900246
M3 - Article
C2 - 31688977
AN - SCOPUS:85075990539
SN - 1864-063X
VL - 13
JO - Journal of biophotonics
JF - Journal of biophotonics
IS - 2
M1 - e201900246
ER -