Morphologic and immunocytochemical performances of effusion cell blocks prepared using 3 different methods

Xin Jing, Qing Kay Li, Ursula Bedrossian, Claire W. Michael

Research output: Contribution to journalArticle

Abstract

With increased use of the ThinPrep method for nongynecologic specimens, cell blocks are more commonly prepared by harvesting cells that are fixed in CytoLyt solution. The current study compared morphologic and immunocytochemical performance of effusion cell blocks prepared using CytoLyt-prefixed thrombin clot (CTC) with plasma thrombin clot (PT) and HistoGel (HG) preparation. The study included a total of 25 malignant or benign serous fluids. Three individual cell block materials were simultaneously prepared from each of the 25 effusion specimens using the CTC, PT, or HG method. HandE staining and immunostaining for pancytokeratin (pan-CK), carcinoembryonic antigen (CEA), epithelial membrane antigen (EMA), B72.3, HBME-1, estrogen receptor (ER), progesterone receptor (PR), CD45, CD20, and CD3 were then performed. The CTC preparation revealed compatible cellularity and good cellular details. In addition, CTC cell blocks revealed a similar percentage of cells with positive immunostaining along with the strongest intensity and the least background staining. The CTC method can be used reliably as an adjunct to other preparation techniques.

Original languageEnglish (US)
Pages (from-to)177-182
Number of pages6
JournalAmerican Journal of Clinical Pathology
Volume139
Issue number2
DOIs
StatePublished - Feb 2013

Fingerprint

Thrombin
Staining and Labeling
Mucin-1
Carcinoembryonic Antigen
Progesterone Receptors
Estrogen Receptors

Keywords

  • Cell block preparations
  • Immunocytochemical performance
  • Morphologic performance
  • Serous effusions

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Morphologic and immunocytochemical performances of effusion cell blocks prepared using 3 different methods. / Jing, Xin; Li, Qing Kay; Bedrossian, Ursula; Michael, Claire W.

In: American Journal of Clinical Pathology, Vol. 139, No. 2, 02.2013, p. 177-182.

Research output: Contribution to journalArticle

@article{103b914330924ab0975575ac16be5e39,
title = "Morphologic and immunocytochemical performances of effusion cell blocks prepared using 3 different methods",
abstract = "With increased use of the ThinPrep method for nongynecologic specimens, cell blocks are more commonly prepared by harvesting cells that are fixed in CytoLyt solution. The current study compared morphologic and immunocytochemical performance of effusion cell blocks prepared using CytoLyt-prefixed thrombin clot (CTC) with plasma thrombin clot (PT) and HistoGel (HG) preparation. The study included a total of 25 malignant or benign serous fluids. Three individual cell block materials were simultaneously prepared from each of the 25 effusion specimens using the CTC, PT, or HG method. HandE staining and immunostaining for pancytokeratin (pan-CK), carcinoembryonic antigen (CEA), epithelial membrane antigen (EMA), B72.3, HBME-1, estrogen receptor (ER), progesterone receptor (PR), CD45, CD20, and CD3 were then performed. The CTC preparation revealed compatible cellularity and good cellular details. In addition, CTC cell blocks revealed a similar percentage of cells with positive immunostaining along with the strongest intensity and the least background staining. The CTC method can be used reliably as an adjunct to other preparation techniques.",
keywords = "Cell block preparations, Immunocytochemical performance, Morphologic performance, Serous effusions",
author = "Xin Jing and Li, {Qing Kay} and Ursula Bedrossian and Michael, {Claire W.}",
year = "2013",
month = "2",
doi = "10.1309/AJCP83ADULCXMAIX",
language = "English (US)",
volume = "139",
pages = "177--182",
journal = "American Journal of Clinical Pathology",
issn = "0002-9173",
publisher = "American Society of Clinical Pathologists",
number = "2",

}

TY - JOUR

T1 - Morphologic and immunocytochemical performances of effusion cell blocks prepared using 3 different methods

AU - Jing, Xin

AU - Li, Qing Kay

AU - Bedrossian, Ursula

AU - Michael, Claire W.

PY - 2013/2

Y1 - 2013/2

N2 - With increased use of the ThinPrep method for nongynecologic specimens, cell blocks are more commonly prepared by harvesting cells that are fixed in CytoLyt solution. The current study compared morphologic and immunocytochemical performance of effusion cell blocks prepared using CytoLyt-prefixed thrombin clot (CTC) with plasma thrombin clot (PT) and HistoGel (HG) preparation. The study included a total of 25 malignant or benign serous fluids. Three individual cell block materials were simultaneously prepared from each of the 25 effusion specimens using the CTC, PT, or HG method. HandE staining and immunostaining for pancytokeratin (pan-CK), carcinoembryonic antigen (CEA), epithelial membrane antigen (EMA), B72.3, HBME-1, estrogen receptor (ER), progesterone receptor (PR), CD45, CD20, and CD3 were then performed. The CTC preparation revealed compatible cellularity and good cellular details. In addition, CTC cell blocks revealed a similar percentage of cells with positive immunostaining along with the strongest intensity and the least background staining. The CTC method can be used reliably as an adjunct to other preparation techniques.

AB - With increased use of the ThinPrep method for nongynecologic specimens, cell blocks are more commonly prepared by harvesting cells that are fixed in CytoLyt solution. The current study compared morphologic and immunocytochemical performance of effusion cell blocks prepared using CytoLyt-prefixed thrombin clot (CTC) with plasma thrombin clot (PT) and HistoGel (HG) preparation. The study included a total of 25 malignant or benign serous fluids. Three individual cell block materials were simultaneously prepared from each of the 25 effusion specimens using the CTC, PT, or HG method. HandE staining and immunostaining for pancytokeratin (pan-CK), carcinoembryonic antigen (CEA), epithelial membrane antigen (EMA), B72.3, HBME-1, estrogen receptor (ER), progesterone receptor (PR), CD45, CD20, and CD3 were then performed. The CTC preparation revealed compatible cellularity and good cellular details. In addition, CTC cell blocks revealed a similar percentage of cells with positive immunostaining along with the strongest intensity and the least background staining. The CTC method can be used reliably as an adjunct to other preparation techniques.

KW - Cell block preparations

KW - Immunocytochemical performance

KW - Morphologic performance

KW - Serous effusions

UR - http://www.scopus.com/inward/record.url?scp=84875071366&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84875071366&partnerID=8YFLogxK

U2 - 10.1309/AJCP83ADULCXMAIX

DO - 10.1309/AJCP83ADULCXMAIX

M3 - Article

C2 - 23355202

AN - SCOPUS:84875071366

VL - 139

SP - 177

EP - 182

JO - American Journal of Clinical Pathology

JF - American Journal of Clinical Pathology

SN - 0002-9173

IS - 2

ER -