Morphogenetic signals from chondrocytes promote chondrogenic and osteogenic differentiation of mesenchymal stem cells

Nathaniel S. Hwang, Shyni Varghese, Christopher Puleo, Zijun Zhang, Jennifer Hartt Elisseeff

Research output: Contribution to journalArticle

Abstract

Mesenchymal stem cells (MSCs) are potentially useful cells for musculoskeletal tissue engineering. However, controlling MSC differentiation and tissue formation in vivo remains a challenge. There is a significant need for well-defined and efficient protocols for directing MSC behaviors in vivo. We hypothesize that morphogenetic signals from chondrocytes may regulate MSC differentiation. In micromass culture of MSCs, incubation with chondrocyte-conditioned medium (CCM) significantly enhanced the production of cartilage specific matrix including type II collagen. In addition, incubation of MSCs with conditioned medium supplemented with osteogenic factors induced more osteogenesis and accumulation of calcium and increased ALP activity. These findings reveal that chondrocyte-secreted factors promote chondrogenesis as well as osteogenesis of MSCs during in vitro micromass culture. Moreover, when MSCs expanded with chondrocyte-conditioned medium were encapsulated in hydrogels and subsequently implanted into athymic mice, basophilic extracellular matrix deposition characteristic of neocartilage was evident. These results indicate that articular chondrocytes produce suitable morphogenetic factors that induce the differentiation program of MSCs in vitro and in vivo.

Original languageEnglish (US)
Pages (from-to)281-284
Number of pages4
JournalJournal of Cellular Physiology
Volume212
Issue number2
DOIs
StatePublished - Aug 2007

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Chondrocytes
Stem cells
Mesenchymal Stromal Cells
Conditioned Culture Medium
Cell culture
Osteogenesis
Cell Differentiation
Chondrogenesis
Hydrogels
Collagen Type II
Cartilage
Tissue Engineering
Tissue engineering
Nude Mice
Extracellular Matrix
Joints
Tissue
Calcium

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Morphogenetic signals from chondrocytes promote chondrogenic and osteogenic differentiation of mesenchymal stem cells. / Hwang, Nathaniel S.; Varghese, Shyni; Puleo, Christopher; Zhang, Zijun; Elisseeff, Jennifer Hartt.

In: Journal of Cellular Physiology, Vol. 212, No. 2, 08.2007, p. 281-284.

Research output: Contribution to journalArticle

Hwang, Nathaniel S. ; Varghese, Shyni ; Puleo, Christopher ; Zhang, Zijun ; Elisseeff, Jennifer Hartt. / Morphogenetic signals from chondrocytes promote chondrogenic and osteogenic differentiation of mesenchymal stem cells. In: Journal of Cellular Physiology. 2007 ; Vol. 212, No. 2. pp. 281-284.
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