Abstract
Polyvalent antisera obtained after immunization of rabbits with crystallins from whole human lens, were further purified by elution of the bound antibody from selected polypeptide bands on a Western blot. These polypeptide bands were either crystallin polypeptides, or their proteolytic fragments. The resulting eluted antisera were monospecific for polypeptides containing immunologic determinants directed towards alpha, beta or gamma crystallins, and could be used in Western blot analysis to characterize their involvement in covalent aggregation occurring in the aging human lens.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 221-232 |
| Number of pages | 12 |
| Journal | Lens Research |
| Volume | 2 |
| Issue number | 3 |
| State | Published - 1984 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Ophthalmology
Cite this
Monospecific antisera to human lens crystallins : Preparation and its use in Western blot analysis. / Takemoto, L. J.; Hansen, J. S.; Zigler, J. S.; Horwitz, J.
In: Lens Research, Vol. 2, No. 3, 1984, p. 221-232.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Monospecific antisera to human lens crystallins
T2 - Preparation and its use in Western blot analysis
AU - Takemoto, L. J.
AU - Hansen, J. S.
AU - Zigler, J. S.
AU - Horwitz, J.
PY - 1984
Y1 - 1984
N2 - Polyvalent antisera obtained after immunization of rabbits with crystallins from whole human lens, were further purified by elution of the bound antibody from selected polypeptide bands on a Western blot. These polypeptide bands were either crystallin polypeptides, or their proteolytic fragments. The resulting eluted antisera were monospecific for polypeptides containing immunologic determinants directed towards alpha, beta or gamma crystallins, and could be used in Western blot analysis to characterize their involvement in covalent aggregation occurring in the aging human lens.
AB - Polyvalent antisera obtained after immunization of rabbits with crystallins from whole human lens, were further purified by elution of the bound antibody from selected polypeptide bands on a Western blot. These polypeptide bands were either crystallin polypeptides, or their proteolytic fragments. The resulting eluted antisera were monospecific for polypeptides containing immunologic determinants directed towards alpha, beta or gamma crystallins, and could be used in Western blot analysis to characterize their involvement in covalent aggregation occurring in the aging human lens.
UR - http://www.scopus.com/inward/record.url?scp=0021724269&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0021724269&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0021724269
VL - 2
SP - 221
EP - 232
JO - Lens Research
JF - Lens Research
SN - 0738-1441
IS - 3
ER -