Monospecific antisera to human lens crystallins: Preparation and its use in Western blot analysis

L. J. Takemoto, J. S. Hansen, J. S. Zigler, J. Horwitz

Research output: Contribution to journalArticle

Abstract

Polyvalent antisera obtained after immunization of rabbits with crystallins from whole human lens, were further purified by elution of the bound antibody from selected polypeptide bands on a Western blot. These polypeptide bands were either crystallin polypeptides, or their proteolytic fragments. The resulting eluted antisera were monospecific for polypeptides containing immunologic determinants directed towards alpha, beta or gamma crystallins, and could be used in Western blot analysis to characterize their involvement in covalent aggregation occurring in the aging human lens.

Original languageEnglish (US)
Pages (from-to)221-232
Number of pages12
JournalLens Research
Volume2
Issue number3
StatePublished - 1984
Externally publishedYes

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Crystallins
Lenses
Immune Sera
Western Blotting
Peptides
beta-Crystallins
gamma-Crystallins
alpha-Crystallins
Immunization
Rabbits
Antibodies

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Takemoto, L. J., Hansen, J. S., Zigler, J. S., & Horwitz, J. (1984). Monospecific antisera to human lens crystallins: Preparation and its use in Western blot analysis. Lens Research, 2(3), 221-232.

Monospecific antisera to human lens crystallins : Preparation and its use in Western blot analysis. / Takemoto, L. J.; Hansen, J. S.; Zigler, J. S.; Horwitz, J.

In: Lens Research, Vol. 2, No. 3, 1984, p. 221-232.

Research output: Contribution to journalArticle

Takemoto, LJ, Hansen, JS, Zigler, JS & Horwitz, J 1984, 'Monospecific antisera to human lens crystallins: Preparation and its use in Western blot analysis', Lens Research, vol. 2, no. 3, pp. 221-232.
Takemoto, L. J. ; Hansen, J. S. ; Zigler, J. S. ; Horwitz, J. / Monospecific antisera to human lens crystallins : Preparation and its use in Western blot analysis. In: Lens Research. 1984 ; Vol. 2, No. 3. pp. 221-232.
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