Monospecific antisera to human lens crystallins: Preparation and its use in Western blot analysis

L. J. Takemoto; J. S. Hansen; J. S. Zigler; J. Horwitz

Monospecific antisera to human lens crystallins: Preparation and its use in Western blot analysis

L. J. Takemoto, J. S. Hansen, J. S. Zigler, J. Horwitz

School of Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

Polyvalent antisera obtained after immunization of rabbits with crystallins from whole human lens, were further purified by elution of the bound antibody from selected polypeptide bands on a Western blot. These polypeptide bands were either crystallin polypeptides, or their proteolytic fragments. The resulting eluted antisera were monospecific for polypeptides containing immunologic determinants directed towards alpha, beta or gamma crystallins, and could be used in Western blot analysis to characterize their involvement in covalent aggregation occurring in the aging human lens.

Original language	English (US)
Pages (from-to)	221-232
Number of pages	12
Journal	Lens Research
Volume	2
Issue number	3
State	Published - Jan 1 1984

ASJC Scopus subject areas

Ophthalmology

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title = "Monospecific antisera to human lens crystallins: Preparation and its use in Western blot analysis",

abstract = "Polyvalent antisera obtained after immunization of rabbits with crystallins from whole human lens, were further purified by elution of the bound antibody from selected polypeptide bands on a Western blot. These polypeptide bands were either crystallin polypeptides, or their proteolytic fragments. The resulting eluted antisera were monospecific for polypeptides containing immunologic determinants directed towards alpha, beta or gamma crystallins, and could be used in Western blot analysis to characterize their involvement in covalent aggregation occurring in the aging human lens.",

author = "Takemoto, {L. J.} and Hansen, {J. S.} and Zigler, {J. S.} and J. Horwitz",

year = "1984",

month = jan,

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language = "English (US)",

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journal = "Lens Research",

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T2 - Preparation and its use in Western blot analysis

AU - Takemoto, L. J.

AU - Hansen, J. S.

AU - Zigler, J. S.

AU - Horwitz, J.

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N2 - Polyvalent antisera obtained after immunization of rabbits with crystallins from whole human lens, were further purified by elution of the bound antibody from selected polypeptide bands on a Western blot. These polypeptide bands were either crystallin polypeptides, or their proteolytic fragments. The resulting eluted antisera were monospecific for polypeptides containing immunologic determinants directed towards alpha, beta or gamma crystallins, and could be used in Western blot analysis to characterize their involvement in covalent aggregation occurring in the aging human lens.

AB - Polyvalent antisera obtained after immunization of rabbits with crystallins from whole human lens, were further purified by elution of the bound antibody from selected polypeptide bands on a Western blot. These polypeptide bands were either crystallin polypeptides, or their proteolytic fragments. The resulting eluted antisera were monospecific for polypeptides containing immunologic determinants directed towards alpha, beta or gamma crystallins, and could be used in Western blot analysis to characterize their involvement in covalent aggregation occurring in the aging human lens.

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