Monocytes are required to trigger Ca2+ uptake in the proliferative response of human T lymphocytes to Staphylococcus aureus protein A

H. M. Lederman, J. W.W. Lee, R. K. Cheung, S. Grinstein, E. W. Gelfand

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Abstract

We have used the T-cell mitogen Staphylococcus aureus protein A (SpA) to study the role of monocytes in the early events of T-lymphocyte activation. The mitogenic response of human peripheral blood mononuclear cells (PBM) was compared to the response of populations enriched for T cells by E-rosetting (PBM-E+). In response to SpA, the [3H]thymidine uptake of PBM-E+ was reduced by 80% compared to PBM. The reduced response of PBM-E+ was completely restored by the addition of irradiated PBM-E- or the monocyte-like human cell line U-937 but not by addition of irradiated PBM-E+. A direct interaction of SpA with monocytes is important since proliferative responses could be generated by preincubation of U-937 with SpA followed by washing and subsequent addition to PBM-E+; incubation of PBM-E+ with SpA followed by washing and subsequent addition of U-937 did not result in a proliferative response. To further delineate the role of the monocyte, we examined the ability of soluble SpA, U-937, or U-937 preincubated with SpA to trigger Ca2+ flux into T lymphocytes, an early step in initiation of the proliferative response. SpA-pretreated U-937, but neither SpA nor monocytes alone, triggered Ca2+ movement into the T lymphocytes. This defines a new role for the monocyte in the early events of T-lymphocyte activation.

Original languageEnglish (US)
Pages (from-to)6827-6830
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume81
Issue number21 I
DOIs
StatePublished - Dec 1 1984
Externally publishedYes

ASJC Scopus subject areas

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