Monocyte enhancers are highly altered in systemic lupus erythematosus

Lihua Shi, Zhe Zhang, Li Song, Yiu Tak Leung, Michelle A. Petri, Kathleen E. Sullivan

Research output: Contribution to journalArticle

Abstract

Objective: Histone modifications set transcriptional competency and can perpetuate pathologic expression patterns. We defined systemic lupus erythematosus (SLE)-specific changes in H3K4me3 and K3K27me3, histone marks of gene activation and repression, respectively. Methods: We used ChIP-seq to define histone modifications in monocytes from SLE patients and controls. Results: Both promoters and enhancers exhibited significant changes in histone methylation in SLE. Regions with differential H3K4me3 in SLE were significantly enriched in potential interferon-related transcription factor binding sites and pioneer transcription factor sites. Conclusion: Enhancer activation defines the character of the cell and our data support extensive disease effects in monocytes, a particularly plastic lineage. Type I interferons not only drive altered gene expression but may also alter the character of the cell through chromatin modifications.

Original languageEnglish (US)
Pages (from-to)921-935
Number of pages15
JournalEpigenomics
Volume7
Issue number6
DOIs
StatePublished - Sep 2015

Keywords

  • IRF1
  • enhancer
  • epigenome
  • histone methylation
  • interferon
  • lupus

ASJC Scopus subject areas

  • Genetics
  • Cancer Research

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  • Cite this

    Shi, L., Zhang, Z., Song, L., Leung, Y. T., Petri, M. A., & Sullivan, K. E. (2015). Monocyte enhancers are highly altered in systemic lupus erythematosus. Epigenomics, 7(6), 921-935. https://doi.org/10.2217/epi.15.47