Monocyte enhancers are highly altered in systemic lupus erythematosus

Lihua Shi, Zhe Zhang, Li Song, Yiu Tak Leung, Michelle Petri, Kathleen E. Sullivan

Research output: Contribution to journalArticle

Abstract

Objective: Histone modifications set transcriptional competency and can perpetuate pathologic expression patterns. We defined systemic lupus erythematosus (SLE)-specific changes in H3K4me3 and K3K27me3, histone marks of gene activation and repression, respectively. Methods: We used ChIP-seq to define histone modifications in monocytes from SLE patients and controls. Results: Both promoters and enhancers exhibited significant changes in histone methylation in SLE. Regions with differential H3K4me3 in SLE were significantly enriched in potential interferon-related transcription factor binding sites and pioneer transcription factor sites. Conclusion: Enhancer activation defines the character of the cell and our data support extensive disease effects in monocytes, a particularly plastic lineage. Type I interferons not only drive altered gene expression but may also alter the character of the cell through chromatin modifications.

Original languageEnglish (US)
Pages (from-to)921-935
Number of pages15
JournalEpigenomics
Volume7
Issue number6
DOIs
StatePublished - Sep 1 2015

Fingerprint

Histone Code
Systemic Lupus Erythematosus
Monocytes
Transcription Factors
Interferon Type I
Histones
Interferons
Methylation
Transcriptional Activation
Plastics
Chromatin
Binding Sites
Gene Expression

Keywords

  • enhancer
  • epigenome
  • histone methylation
  • interferon
  • IRF1
  • lupus

ASJC Scopus subject areas

  • Genetics
  • Cancer Research

Cite this

Shi, L., Zhang, Z., Song, L., Leung, Y. T., Petri, M., & Sullivan, K. E. (2015). Monocyte enhancers are highly altered in systemic lupus erythematosus. Epigenomics, 7(6), 921-935. https://doi.org/10.2217/epi.15.47

Monocyte enhancers are highly altered in systemic lupus erythematosus. / Shi, Lihua; Zhang, Zhe; Song, Li; Leung, Yiu Tak; Petri, Michelle; Sullivan, Kathleen E.

In: Epigenomics, Vol. 7, No. 6, 01.09.2015, p. 921-935.

Research output: Contribution to journalArticle

Shi, L, Zhang, Z, Song, L, Leung, YT, Petri, M & Sullivan, KE 2015, 'Monocyte enhancers are highly altered in systemic lupus erythematosus', Epigenomics, vol. 7, no. 6, pp. 921-935. https://doi.org/10.2217/epi.15.47
Shi, Lihua ; Zhang, Zhe ; Song, Li ; Leung, Yiu Tak ; Petri, Michelle ; Sullivan, Kathleen E. / Monocyte enhancers are highly altered in systemic lupus erythematosus. In: Epigenomics. 2015 ; Vol. 7, No. 6. pp. 921-935.
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