Abstract
Objective: Histone modifications set transcriptional competency and can perpetuate pathologic expression patterns. We defined systemic lupus erythematosus (SLE)-specific changes in H3K4me3 and K3K27me3, histone marks of gene activation and repression, respectively. Methods: We used ChIP-seq to define histone modifications in monocytes from SLE patients and controls. Results: Both promoters and enhancers exhibited significant changes in histone methylation in SLE. Regions with differential H3K4me3 in SLE were significantly enriched in potential interferon-related transcription factor binding sites and pioneer transcription factor sites. Conclusion: Enhancer activation defines the character of the cell and our data support extensive disease effects in monocytes, a particularly plastic lineage. Type I interferons not only drive altered gene expression but may also alter the character of the cell through chromatin modifications.
Original language | English (US) |
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Pages (from-to) | 921-935 |
Number of pages | 15 |
Journal | Epigenomics |
Volume | 7 |
Issue number | 6 |
DOIs | |
State | Published - Sep 2015 |
Keywords
- IRF1
- enhancer
- epigenome
- histone methylation
- interferon
- lupus
ASJC Scopus subject areas
- Genetics
- Cancer Research