Monoclonal antibody specific for T cell-derived human IgE binding factors

T. Kisaki, T. F. Huff, D. H. Conrad, J. Yodoi, K. Ishizaka

Research output: Contribution to journalArticlepeer-review

Abstract

A B cell hybridoma secreting monoclonal antibody against human IgE binding factors was obtained by immunization of BALB/c mice with partially purified IgE binding factors, and fusion of their spleen cells with SP-2/0-AG14 cells. The monoclonal antibody bound all of the 60,000, 30,000, and 15,000 dalton IgE binding factors from two T cell hybridomas and those from activated T cells of a normal individual. The antibody bound both IgE-potentiating factors, which had affinity for lentil lectin, and IgE-suppressive factors, which had affinity for peanut agglutinin. However, the monoclonal anti-IgE-binding factor bound neither FcεR on RPMI 8866 cells nor IgE binding factors from the B lymphoblastoid cells. A monoclonal antibody against FcεR on B cells (H107) bound the 60,000 and 30,000 dalton IgE binding factors from both T cell hybridomas and RPMI 8866 cells but did not bind the 15,000 dalton IgE binding factors from either T cells or B cells. The results indicate that T cell-derived IgE binding factors have a unique antigenic determinant that is lacking in both FcεR on B cells and B cell-derived IgE binding factors. The anti-IgE binding factor and anti-FcεR monoclonal antibodies both failed to stain cell surface components of IgE binding factor-producing T cell hybridomas. However, both antibodies induced the T cell hybridoma to form IgE binding factors. The results suggest that the T cell hybridomas bear low numbers of FcεR that share antigenic determinants with IgE binding factors secreted from the cells.

Original languageEnglish (US)
Pages (from-to)3345-3351
Number of pages7
JournalJournal of Immunology
Volume138
Issue number10
StatePublished - 1987

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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