Monoclonal antibody specific for T cell-derived human IgE binding factors

T. Kisaki, T. F. Huff, D. H. Conrad, J. Yodoi, K. Ishizaka

Research output: Contribution to journalArticle

Abstract

A B cell hybridoma secreting monoclonal antibody against human IgE binding factors was obtained by immunization of BALB/c mice with partially purified IgE binding factors, and fusion of their spleen cells with SP-2/0-AG14 cells. The monoclonal antibody bound all of the 60,000, 30,000, and 15,000 dalton IgE binding factors from two T cell hybridomas and those from activated T cells of a normal individual. The antibody bound both IgE-potentiating factors, which had affinity for lentil lectin, and IgE-suppressive factors, which had affinity for peanut agglutinin. However, the monoclonal anti-IgE-binding factor bound neither FcεR on RPMI 8866 cells nor IgE binding factors from the B lymphoblastoid cells. A monoclonal antibody against FcεR on B cells (H107) bound the 60,000 and 30,000 dalton IgE binding factors from both T cell hybridomas and RPMI 8866 cells but did not bind the 15,000 dalton IgE binding factors from either T cells or B cells. The results indicate that T cell-derived IgE binding factors have a unique antigenic determinant that is lacking in both FcεR on B cells and B cell-derived IgE binding factors. The anti-IgE binding factor and anti-FcεR monoclonal antibodies both failed to stain cell surface components of IgE binding factor-producing T cell hybridomas. However, both antibodies induced the T cell hybridoma to form IgE binding factors. The results suggest that the T cell hybridomas bear low numbers of FcεR that share antigenic determinants with IgE binding factors secreted from the cells.

Original languageEnglish (US)
Pages (from-to)3345-3351
Number of pages7
JournalJournal of Immunology
Volume138
Issue number10
StatePublished - 1987
Externally publishedYes

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Monoclonal Antibodies
T-Lymphocytes
Hybridomas
B-Lymphocytes
immunoglobulin-binding factors
Epitopes
Peanut Agglutinin
Antibodies
Cellular Structures
Immunoglobulin E
Immunization
Coloring Agents
Spleen

ASJC Scopus subject areas

  • Immunology

Cite this

Kisaki, T., Huff, T. F., Conrad, D. H., Yodoi, J., & Ishizaka, K. (1987). Monoclonal antibody specific for T cell-derived human IgE binding factors. Journal of Immunology, 138(10), 3345-3351.

Monoclonal antibody specific for T cell-derived human IgE binding factors. / Kisaki, T.; Huff, T. F.; Conrad, D. H.; Yodoi, J.; Ishizaka, K.

In: Journal of Immunology, Vol. 138, No. 10, 1987, p. 3345-3351.

Research output: Contribution to journalArticle

Kisaki, T, Huff, TF, Conrad, DH, Yodoi, J & Ishizaka, K 1987, 'Monoclonal antibody specific for T cell-derived human IgE binding factors', Journal of Immunology, vol. 138, no. 10, pp. 3345-3351.
Kisaki T, Huff TF, Conrad DH, Yodoi J, Ishizaka K. Monoclonal antibody specific for T cell-derived human IgE binding factors. Journal of Immunology. 1987;138(10):3345-3351.
Kisaki, T. ; Huff, T. F. ; Conrad, D. H. ; Yodoi, J. ; Ishizaka, K. / Monoclonal antibody specific for T cell-derived human IgE binding factors. In: Journal of Immunology. 1987 ; Vol. 138, No. 10. pp. 3345-3351.
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