Monoclonal antibody mediated capsular reactions (Quellung) in Cryptococcus neoformans

Jean Mukherjee; Wendy Cleare; Arturo Casadevall

doi:10.1016/0022-1759(95)00097-T

Monoclonal antibody mediated capsular reactions (Quellung) in Cryptococcus neoformans

Jean Mukherjee, Wendy Cleare, Arturo Casadevall

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

Capsular reactions ('Quellung') visible by light microscopy were observed when capsule-binding monoclonal antibody (mAb) was added to Cryptococcus neoformans yeast cells. Capsular reactions were observed with capsule-binding IgM, IgG1, IgG2a, IgG2b, IgG3 and IgA mAbs. Scanning electron microscopy revealed that mAb binding produced a structural change in the fibrillar network of the capsule. The occurrence of capsular reactions with mAbs indicate that this phenomenon can be produced by the binding of antibody to a single epitope.

Original language	English (US)
Pages (from-to)	139-143
Number of pages	5
Journal	Journal of Immunological Methods
Volume	184
Issue number	1
DOIs	https://doi.org/10.1016/0022-1759(95)00097-T
State	Published - Jul 17 1995
Externally published	Yes

Keywords

AIDS
Capsule
Cryptococcus neoformans
Monoclonal antibody
Polysaccharide

ASJC Scopus subject areas

Immunology and Allergy
Immunology

Access to Document

10.1016/0022-1759(95)00097-T

Cite this

@article{228aee986758453e8f986e048b0c0c9f,

title = "Monoclonal antibody mediated capsular reactions (Quellung) in Cryptococcus neoformans",

abstract = "Capsular reactions ('Quellung') visible by light microscopy were observed when capsule-binding monoclonal antibody (mAb) was added to Cryptococcus neoformans yeast cells. Capsular reactions were observed with capsule-binding IgM, IgG1, IgG2a, IgG2b, IgG3 and IgA mAbs. Scanning electron microscopy revealed that mAb binding produced a structural change in the fibrillar network of the capsule. The occurrence of capsular reactions with mAbs indicate that this phenomenon can be produced by the binding of antibody to a single epitope.",

keywords = "AIDS, Capsule, Cryptococcus neoformans, Monoclonal antibody, Polysaccharide",

author = "Jean Mukherjee and Wendy Cleare and Arturo Casadevall",

note = "Funding Information: J.M. was supportedb y NIH grant 9-526-3684. A.C. and WC. are supported by a James S. McDonnell Scholar Award and NIH grants AI33774 and AI13342.W e thank Dr. MatthewD . Scharff for many helpful discussionsW. e thank Frank Macaluso of the Albert Einstein Cancer Center Analytical Ultrastructure Facility for as-sistancew ith electron microscopy.W e thank Dr. SushovitaM ukherjee for preparationo f some ascites samples.",

year = "1995",

month = jul,

day = "17",

doi = "10.1016/0022-1759(95)00097-T",

language = "English (US)",

volume = "184",

pages = "139--143",

journal = "Journal of Immunological Methods",

issn = "0022-1759",

publisher = "Elsevier",

number = "1",

}

TY - JOUR

T1 - Monoclonal antibody mediated capsular reactions (Quellung) in Cryptococcus neoformans

AU - Mukherjee, Jean

AU - Cleare, Wendy

AU - Casadevall, Arturo

N1 - Funding Information: J.M. was supportedb y NIH grant 9-526-3684. A.C. and WC. are supported by a James S. McDonnell Scholar Award and NIH grants AI33774 and AI13342.W e thank Dr. MatthewD . Scharff for many helpful discussionsW. e thank Frank Macaluso of the Albert Einstein Cancer Center Analytical Ultrastructure Facility for as-sistancew ith electron microscopy.W e thank Dr. SushovitaM ukherjee for preparationo f some ascites samples.

PY - 1995/7/17

Y1 - 1995/7/17

N2 - Capsular reactions ('Quellung') visible by light microscopy were observed when capsule-binding monoclonal antibody (mAb) was added to Cryptococcus neoformans yeast cells. Capsular reactions were observed with capsule-binding IgM, IgG1, IgG2a, IgG2b, IgG3 and IgA mAbs. Scanning electron microscopy revealed that mAb binding produced a structural change in the fibrillar network of the capsule. The occurrence of capsular reactions with mAbs indicate that this phenomenon can be produced by the binding of antibody to a single epitope.

AB - Capsular reactions ('Quellung') visible by light microscopy were observed when capsule-binding monoclonal antibody (mAb) was added to Cryptococcus neoformans yeast cells. Capsular reactions were observed with capsule-binding IgM, IgG1, IgG2a, IgG2b, IgG3 and IgA mAbs. Scanning electron microscopy revealed that mAb binding produced a structural change in the fibrillar network of the capsule. The occurrence of capsular reactions with mAbs indicate that this phenomenon can be produced by the binding of antibody to a single epitope.

KW - AIDS

KW - Capsule

KW - Cryptococcus neoformans

KW - Monoclonal antibody

KW - Polysaccharide

UR - http://www.scopus.com/inward/record.url?scp=0029006253&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029006253&partnerID=8YFLogxK

U2 - 10.1016/0022-1759(95)00097-T

DO - 10.1016/0022-1759(95)00097-T

M3 - Article

C2 - 7622865

AN - SCOPUS:0029006253

SN - 0022-1759

VL - 184

SP - 139

EP - 143

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

IS - 1

ER -

Monoclonal antibody mediated capsular reactions (Quellung) in Cryptococcus neoformans

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this