Monoclonal antibody mediated capsular reactions (Quellung) in Cryptococcus neoformans

Jean Mukherjee, Wendy Cleare, Arturo Casadevall

Research output: Contribution to journalArticle

Abstract

Capsular reactions ('Quellung') visible by light microscopy were observed when capsule-binding monoclonal antibody (mAb) was added to Cryptococcus neoformans yeast cells. Capsular reactions were observed with capsule-binding IgM, IgG1, IgG2a, IgG2b, IgG3 and IgA mAbs. Scanning electron microscopy revealed that mAb binding produced a structural change in the fibrillar network of the capsule. The occurrence of capsular reactions with mAbs indicate that this phenomenon can be produced by the binding of antibody to a single epitope.

Original languageEnglish (US)
Pages (from-to)139-143
Number of pages5
JournalJournal of Immunological Methods
Volume184
Issue number1
DOIs
StatePublished - Jul 17 1995
Externally publishedYes

Keywords

  • AIDS
  • Capsule
  • Cryptococcus neoformans
  • Monoclonal antibody
  • Polysaccharide

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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