Monoclonal antibody localization of A and B isoantigens in normal and malignant fixed human tissues

C. Ernst, J. Thurin, B. Atkinson, H. Wurzel, M. Herlyn, N. Stromberg, C. Civin, H. Koprowski

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Abstract

The expression of human blood group A and B isoantigens in normal and malignant tissues from stomach, colon, and pancreas was analyzed in an immunoperoxidase assay using monoclonal antibodies specific for these isoantigens. Appropriate isoantigen expression was demonstrated in the normal epithelium from the stomach, pancreas, and proximal but not distal colon of blood group A, AB, or B patients. Half of all gastric carcinomas and of proximal colon carcinomas showed complete loss of isoantigen, whereas the adjacent mucosa in these cases continued to express appropriate isoantigen. Isoantigen expression was completely lost in only 13% of pancreatic carcinomas tested. Neither A nor B isoantigen was detected in normal epithelium from the distal colon. By contrast, 85% of carcinomas derived from this site showed reexpression of isoantigen. Inappropriate expression of A isoantigen was detected in pancreatic carcinomas (2/5) but not in gastric or colon carcinoma (0/21). Inappropriate expression of B substance was not detected in any tissue (0/38). Interestingly, differential binding of antibodies to Type 1 versus Type 2 and/or difucosyl versus monofucosyl blood group B substances was manifested by differences in intensity of staining for endothelium and red blood cells.

Original languageEnglish (US)
Pages (from-to)451-461
Number of pages11
JournalAmerican Journal of Pathology
Volume117
Issue number3
Publication statusPublished - 1984
Externally publishedYes

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ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Ernst, C., Thurin, J., Atkinson, B., Wurzel, H., Herlyn, M., Stromberg, N., ... Koprowski, H. (1984). Monoclonal antibody localization of A and B isoantigens in normal and malignant fixed human tissues. American Journal of Pathology, 117(3), 451-461.