Monoclonal antibody JSB‐1 detects a highly conserved epitope on the P‐glycoprotein associated with multi‐drug‐resistance

R. J. Scheper; J. W.M. Bulte; J. G.P. Brakkee; J. J. Quak; E. van der Schoot; A. J.M. Balm; C. J.L.M. Melier; H. J. Broxteman; C. M. Kuiper; J. Lankelma; H. M. Pinedo

doi:10.1002/ijc.2910420314

Monoclonal antibody JSB‐1 detects a highly conserved epitope on the P‐glycoprotein associated with multi‐drug‐resistance

R. J. Scheper, J. W.M. Bulte, J. G.P. Brakkee, J. J. Quak, E. van der Schoot, A. J.M. Balm, C. J.L.M. Melier, H. J. Broxteman, C. M. Kuiper, J. Lankelma, H. M. Pinedo

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Abstract

Resistance to multiple chemotherapeutic agents is a common clinical problem in the treatment of cancer. This resistance may occur before primary therapy or be acquired during treatment. We have generated a monoclonal antibody (MAb) (JSB‐I), specific for a conserved epitope on the plasma membrane 170‐ to 180‐kDa glycoprotein, the expression of which is strongly correlated with the degree of multi‐drug resistance (MDR). JSB‐1 strongly binds to both Chinese‐hamster‐derived MDR cell lines and human MDR cell lines, including cell lines derived from lung and ovary. A drug‐sensitive revertant line, and the corresponding drug‐sensitive parent lines, showed only weak reactivity or none at all. JSS‐l reacts strongly to air‐dried or acetone‐fixed cells and therefore has potential value for diagnostic detection of MDR cells in human tumor samples.

Original language	English (US)
Pages (from-to)	389-394
Number of pages	6
Journal	International Journal of Cancer
Volume	42
Issue number	3
DOIs	https://doi.org/10.1002/ijc.2910420314
State	Published - Sep 15 1988
Externally published	Yes

ASJC Scopus subject areas

Oncology
Cancer Research

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Scheper, R. J., Bulte, J. W. M., Brakkee, J. G. P., Quak, J. J., van der Schoot, E., Balm, A. J. M., Melier, C. J. L. M., Broxteman, H. J., Kuiper, C. M., Lankelma, J., & Pinedo, H. M. (1988). Monoclonal antibody JSB‐1 detects a highly conserved epitope on the P‐glycoprotein associated with multi‐drug‐resistance. International Journal of Cancer, 42(3), 389-394. https://doi.org/10.1002/ijc.2910420314

Scheper, RJ, Bulte, JWM, Brakkee, JGP, Quak, JJ, van der Schoot, E, Balm, AJM, Melier, CJLM, Broxteman, HJ, Kuiper, CM, Lankelma, J & Pinedo, HM 1988, 'Monoclonal antibody JSB‐1 detects a highly conserved epitope on the P‐glycoprotein associated with multi‐drug‐resistance', International Journal of Cancer, vol. 42, no. 3, pp. 389-394. https://doi.org/10.1002/ijc.2910420314

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title = "Monoclonal antibody JSB‐1 detects a highly conserved epitope on the P‐glycoprotein associated with multi‐drug‐resistance",

abstract = "Resistance to multiple chemotherapeutic agents is a common clinical problem in the treatment of cancer. This resistance may occur before primary therapy or be acquired during treatment. We have generated a monoclonal antibody (MAb) (JSB‐I), specific for a conserved epitope on the plasma membrane 170‐ to 180‐kDa glycoprotein, the expression of which is strongly correlated with the degree of multi‐drug resistance (MDR). JSB‐1 strongly binds to both Chinese‐hamster‐derived MDR cell lines and human MDR cell lines, including cell lines derived from lung and ovary. A drug‐sensitive revertant line, and the corresponding drug‐sensitive parent lines, showed only weak reactivity or none at all. JSS‐l reacts strongly to air‐dried or acetone‐fixed cells and therefore has potential value for diagnostic detection of MDR cells in human tumor samples.",

author = "Scheper, {R. J.} and Bulte, {J. W.M.} and Brakkee, {J. G.P.} and Quak, {J. J.} and {van der Schoot}, E. and Balm, {A. J.M.} and Melier, {C. J.L.M.} and Broxteman, {H. J.} and Kuiper, {C. M.} and J. Lankelma and Pinedo, {H. M.}",

year = "1988",

month = sep,

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doi = "10.1002/ijc.2910420314",

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AU - Scheper, R. J.

AU - Bulte, J. W.M.

AU - Brakkee, J. G.P.

AU - Quak, J. J.

AU - van der Schoot, E.

AU - Balm, A. J.M.

AU - Melier, C. J.L.M.

AU - Broxteman, H. J.

AU - Kuiper, C. M.

AU - Lankelma, J.

AU - Pinedo, H. M.

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AB - Resistance to multiple chemotherapeutic agents is a common clinical problem in the treatment of cancer. This resistance may occur before primary therapy or be acquired during treatment. We have generated a monoclonal antibody (MAb) (JSB‐I), specific for a conserved epitope on the plasma membrane 170‐ to 180‐kDa glycoprotein, the expression of which is strongly correlated with the degree of multi‐drug resistance (MDR). JSB‐1 strongly binds to both Chinese‐hamster‐derived MDR cell lines and human MDR cell lines, including cell lines derived from lung and ovary. A drug‐sensitive revertant line, and the corresponding drug‐sensitive parent lines, showed only weak reactivity or none at all. JSS‐l reacts strongly to air‐dried or acetone‐fixed cells and therefore has potential value for diagnostic detection of MDR cells in human tumor samples.

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Monoclonal antibody JSB‐1 detects a highly conserved epitope on the P‐glycoprotein associated with multi‐drug‐resistance

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