Monoclonal antibodies to the enterotoxin of Bacteroides fragilis: Production, characterization, and immunodiagnosis application

Firdausi Qadri, M. Golam Mohi, Ashrafuzzaman Chowdhury, Khorshid Alam, Tasnim Azim, Cynthia Louise Sears, R. Bradley Sack, R. John Albert

Research output: Contribution to journalArticle

Abstract

A monoclonal antibody, ICT11, specific for the toxin of enterotoxigenic Bacteroides fragilis (ETBF) neutralized the cytotoxic effect of the toxin on human colonic cell line HT-29/C1. In an evaluation using 115 diarrheal stool specimens and culture as the 'gold standard,' the assay showed a sensitivity of 85% and a specificity of 100%. An ICT11-based sandwich enzyme-linked immunosorbent assay showed a sensitivity of 100% and a specificity of 98% for direct detection of toxin from stool samples compared with those of culture. Thus, ICT11-based assays will be useful for screening for ETBF.

Original languageEnglish (US)
Pages (from-to)608-610
Number of pages3
JournalClinical and Diagnostic Laboratory Immunology
Volume3
Issue number5
StatePublished - 1996

Fingerprint

Bacteroides fragilis
Immunologic Tests
Assays
Enzyme-Linked Immunosorbent Assay
Monoclonal Antibodies
Cell Line
Immunosorbents
Screening
Cells
Enzymes
Bacteroides fragilis toxin
fragilysin

ASJC Scopus subject areas

  • Microbiology (medical)
  • Immunology and Allergy
  • Clinical Biochemistry
  • Immunology

Cite this

Monoclonal antibodies to the enterotoxin of Bacteroides fragilis : Production, characterization, and immunodiagnosis application. / Qadri, Firdausi; Mohi, M. Golam; Chowdhury, Ashrafuzzaman; Alam, Khorshid; Azim, Tasnim; Sears, Cynthia Louise; Sack, R. Bradley; Albert, R. John.

In: Clinical and Diagnostic Laboratory Immunology, Vol. 3, No. 5, 1996, p. 608-610.

Research output: Contribution to journalArticle

Qadri, Firdausi ; Mohi, M. Golam ; Chowdhury, Ashrafuzzaman ; Alam, Khorshid ; Azim, Tasnim ; Sears, Cynthia Louise ; Sack, R. Bradley ; Albert, R. John. / Monoclonal antibodies to the enterotoxin of Bacteroides fragilis : Production, characterization, and immunodiagnosis application. In: Clinical and Diagnostic Laboratory Immunology. 1996 ; Vol. 3, No. 5. pp. 608-610.
@article{7adfa5ae73e241bcb9ee1ec0d6565782,
title = "Monoclonal antibodies to the enterotoxin of Bacteroides fragilis: Production, characterization, and immunodiagnosis application",
abstract = "A monoclonal antibody, ICT11, specific for the toxin of enterotoxigenic Bacteroides fragilis (ETBF) neutralized the cytotoxic effect of the toxin on human colonic cell line HT-29/C1. In an evaluation using 115 diarrheal stool specimens and culture as the 'gold standard,' the assay showed a sensitivity of 85{\%} and a specificity of 100{\%}. An ICT11-based sandwich enzyme-linked immunosorbent assay showed a sensitivity of 100{\%} and a specificity of 98{\%} for direct detection of toxin from stool samples compared with those of culture. Thus, ICT11-based assays will be useful for screening for ETBF.",
author = "Firdausi Qadri and Mohi, {M. Golam} and Ashrafuzzaman Chowdhury and Khorshid Alam and Tasnim Azim and Sears, {Cynthia Louise} and Sack, {R. Bradley} and Albert, {R. John}",
year = "1996",
language = "English (US)",
volume = "3",
pages = "608--610",
journal = "Clinical and Vaccine Immunology",
issn = "1556-6811",
publisher = "American Society for Microbiology",
number = "5",

}

TY - JOUR

T1 - Monoclonal antibodies to the enterotoxin of Bacteroides fragilis

T2 - Production, characterization, and immunodiagnosis application

AU - Qadri, Firdausi

AU - Mohi, M. Golam

AU - Chowdhury, Ashrafuzzaman

AU - Alam, Khorshid

AU - Azim, Tasnim

AU - Sears, Cynthia Louise

AU - Sack, R. Bradley

AU - Albert, R. John

PY - 1996

Y1 - 1996

N2 - A monoclonal antibody, ICT11, specific for the toxin of enterotoxigenic Bacteroides fragilis (ETBF) neutralized the cytotoxic effect of the toxin on human colonic cell line HT-29/C1. In an evaluation using 115 diarrheal stool specimens and culture as the 'gold standard,' the assay showed a sensitivity of 85% and a specificity of 100%. An ICT11-based sandwich enzyme-linked immunosorbent assay showed a sensitivity of 100% and a specificity of 98% for direct detection of toxin from stool samples compared with those of culture. Thus, ICT11-based assays will be useful for screening for ETBF.

AB - A monoclonal antibody, ICT11, specific for the toxin of enterotoxigenic Bacteroides fragilis (ETBF) neutralized the cytotoxic effect of the toxin on human colonic cell line HT-29/C1. In an evaluation using 115 diarrheal stool specimens and culture as the 'gold standard,' the assay showed a sensitivity of 85% and a specificity of 100%. An ICT11-based sandwich enzyme-linked immunosorbent assay showed a sensitivity of 100% and a specificity of 98% for direct detection of toxin from stool samples compared with those of culture. Thus, ICT11-based assays will be useful for screening for ETBF.

UR - http://www.scopus.com/inward/record.url?scp=0029793566&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029793566&partnerID=8YFLogxK

M3 - Article

C2 - 8877146

AN - SCOPUS:0029793566

VL - 3

SP - 608

EP - 610

JO - Clinical and Vaccine Immunology

JF - Clinical and Vaccine Immunology

SN - 1556-6811

IS - 5

ER -