Monoclonal antibodies to the enterotoxin of Bacteroides fragilis: Production, characterization, and immunodiagnosis application

Firdausi Qadri, M. Golam Mohi, Ashrafuzzaman Chowdhury, Khorshid Alam, Tasnim Azim, Cynthia Sears, R. Bradley Sack, R. John Albert

Research output: Contribution to journalShort surveypeer-review

Abstract

A monoclonal antibody, ICT11, specific for the toxin of enterotoxigenic Bacteroides fragilis (ETBF) neutralized the cytotoxic effect of the toxin on human colonic cell line HT-29/C1. In an evaluation using 115 diarrheal stool specimens and culture as the 'gold standard,' the assay showed a sensitivity of 85% and a specificity of 100%. An ICT11-based sandwich enzyme-linked immunosorbent assay showed a sensitivity of 100% and a specificity of 98% for direct detection of toxin from stool samples compared with those of culture. Thus, ICT11-based assays will be useful for screening for ETBF.

Original languageEnglish (US)
Pages (from-to)608-610
Number of pages3
JournalClinical and Diagnostic Laboratory Immunology
Volume3
Issue number5
DOIs
StatePublished - 1996

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Clinical Biochemistry
  • Microbiology (medical)

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