TY - JOUR
T1 - Monoclonal antibodies to the enterotoxin of Bacteroides fragilis
T2 - Production, characterization, and immunodiagnosis application
AU - Qadri, Firdausi
AU - Mohi, M. Golam
AU - Chowdhury, Ashrafuzzaman
AU - Alam, Khorshid
AU - Azim, Tasnim
AU - Sears, Cynthia
AU - Sack, R. Bradley
AU - Albert, R. John
PY - 1996
Y1 - 1996
N2 - A monoclonal antibody, ICT11, specific for the toxin of enterotoxigenic Bacteroides fragilis (ETBF) neutralized the cytotoxic effect of the toxin on human colonic cell line HT-29/C1. In an evaluation using 115 diarrheal stool specimens and culture as the 'gold standard,' the assay showed a sensitivity of 85% and a specificity of 100%. An ICT11-based sandwich enzyme-linked immunosorbent assay showed a sensitivity of 100% and a specificity of 98% for direct detection of toxin from stool samples compared with those of culture. Thus, ICT11-based assays will be useful for screening for ETBF.
AB - A monoclonal antibody, ICT11, specific for the toxin of enterotoxigenic Bacteroides fragilis (ETBF) neutralized the cytotoxic effect of the toxin on human colonic cell line HT-29/C1. In an evaluation using 115 diarrheal stool specimens and culture as the 'gold standard,' the assay showed a sensitivity of 85% and a specificity of 100%. An ICT11-based sandwich enzyme-linked immunosorbent assay showed a sensitivity of 100% and a specificity of 98% for direct detection of toxin from stool samples compared with those of culture. Thus, ICT11-based assays will be useful for screening for ETBF.
UR - http://www.scopus.com/inward/record.url?scp=0029793566&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029793566&partnerID=8YFLogxK
U2 - 10.1128/cdli.3.5.608-610.1996
DO - 10.1128/cdli.3.5.608-610.1996
M3 - Short survey
C2 - 8877146
AN - SCOPUS:0029793566
SN - 1071-412X
VL - 3
SP - 608
EP - 610
JO - Clinical and Diagnostic Laboratory Immunology
JF - Clinical and Diagnostic Laboratory Immunology
IS - 5
ER -