TY - JOUR
T1 - Monoclonal antibodies to gonadotropin-releasing hormone (GnRH) inhibit binding of the hormone to its receptor
AU - Gangatirkar, Pradnya
AU - Gangadharan, Sunil
AU - Narendranath, Anita
AU - Nagpal, Sushma
AU - Salunke, Dinakar M.
AU - Karande, Anjali A.
PY - 2002/1/1
Y1 - 2002/1/1
N2 - Monoclonal antibodies (MAbs) specific to gonadotropin-releasing hormone (GnRH) were obtained using different strategies of conjugation of the peptide to carrier protein and immunization. Of several antibodies obtained, two, namely F1D3C5 and E2D2 bound GnRH in solution phase. Though the epitopes corresponding to the two overlapped, there was a one amino acid shift in the core epitope. These two antibodies were characterized with respect to inhibition of GnRH induced responses in rat pituitary cultures and α-T3.1 mouse gonadotrope cell line.
AB - Monoclonal antibodies (MAbs) specific to gonadotropin-releasing hormone (GnRH) were obtained using different strategies of conjugation of the peptide to carrier protein and immunization. Of several antibodies obtained, two, namely F1D3C5 and E2D2 bound GnRH in solution phase. Though the epitopes corresponding to the two overlapped, there was a one amino acid shift in the core epitope. These two antibodies were characterized with respect to inhibition of GnRH induced responses in rat pituitary cultures and α-T3.1 mouse gonadotrope cell line.
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U2 - 10.1089/153685902760213895
DO - 10.1089/153685902760213895
M3 - Article
C2 - 12193281
AN - SCOPUS:0036374194
SN - 1536-8599
VL - 21
SP - 281
EP - 286
JO - Hybridoma and Hybridomics
JF - Hybridoma and Hybridomics
IS - 4
ER -