Monoclonal antibodies recognize a processing dependent epitope present in the mature CS protein of various plasmodial species

M. Tsuji, G. Corradin, Fidel P Zavala

Research output: Contribution to journalArticle

Abstract

In the present paper, we have characterized the specificity of a series of monoclonal antibodies (MoAbs) against Plasmodium berghei sporozoites, selected for their lack of reactivity with the repeat domain of circumsporozoite (CS) protein. We found that these MoAbs recognize Pb44, the mature membrane form of the CS protein, but they do not react with Pb54, its precursor. Furthermore, these MoAbs do not react with any of the synthetic peptides representing the linear sequence of the P. berghei CS protein nor do they react with a recombinant CS (rCS) protein. These observations indicate that the epitope recognized by these antibodies is expressed only after the processing of the CS protein has occurred. This 'processing dependent epitope' is present in sporozoites of P. berghei, and also in those of P. falciparum, P. yoelii and P. brasilianum. It is not present in sporozoites of the P. cynomolgi-P. vivax complex and of P. gallinaceum. These anti-sporozoite MoAbs strongly inhibit sporozoite invasion of hepatoma cells, in vitro, however, they displayed no protective effect in an in vivo assay.

Original languageEnglish (US)
Pages (from-to)457-469
Number of pages13
JournalParasite Immunology
Volume14
Issue number5
Publication statusPublished - 1992
Externally publishedYes

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Keywords

  • A processing dependent epitope
  • Circumsporozoite protein
  • Monoclonal anti-sporozoite antibodies

ASJC Scopus subject areas

  • Immunology
  • Parasitology

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