Monoclonal antibodies for dengue virus prM glycoprotein protect mice against lethal dengue infection

B. M. Kaufman, P. L. Summers, D. R. Dubois, W. H. Cohen, M. K. Gentry, R. L. Timchak, D. S. Burke, K. H. Eckels

Research output: Contribution to journalArticle

Abstract

Five murine monoclonal antibodies (Mabs) reactive against the prM glycoproteins of DEN-3 and -4 were used to passively protect mice in vivo against lethal challenge with homologous and heterologous dengue virus serotypes. Four of the 5 prM-reactive monoclonals cross-protected mice against heterologous challenge, whereas 1 protected against challenge with only the homologous serotype. Although in vitro binding to virions was readily demonstrated, only 2 of the prM Mabs had detectable neutralizing activity. The neutralizing activity could not be enhanced by anti-mouse immunoglobulin or complement. However, 4 of the 5 prM Mabs fixed complement. This is the first report of prM-specific Mabs that are protective in mice.

Original languageEnglish (US)
Pages (from-to)576-580
Number of pages5
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume41
Issue number5
StatePublished - 1989
Externally publishedYes

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Dengue Virus
Dengue
Glycoproteins
Monoclonal Antibodies
Infection
Virion
Immunoglobulins
Serogroup

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Kaufman, B. M., Summers, P. L., Dubois, D. R., Cohen, W. H., Gentry, M. K., Timchak, R. L., ... Eckels, K. H. (1989). Monoclonal antibodies for dengue virus prM glycoprotein protect mice against lethal dengue infection. American Journal of Tropical Medicine and Hygiene, 41(5), 576-580.

Monoclonal antibodies for dengue virus prM glycoprotein protect mice against lethal dengue infection. / Kaufman, B. M.; Summers, P. L.; Dubois, D. R.; Cohen, W. H.; Gentry, M. K.; Timchak, R. L.; Burke, D. S.; Eckels, K. H.

In: American Journal of Tropical Medicine and Hygiene, Vol. 41, No. 5, 1989, p. 576-580.

Research output: Contribution to journalArticle

Kaufman, BM, Summers, PL, Dubois, DR, Cohen, WH, Gentry, MK, Timchak, RL, Burke, DS & Eckels, KH 1989, 'Monoclonal antibodies for dengue virus prM glycoprotein protect mice against lethal dengue infection', American Journal of Tropical Medicine and Hygiene, vol. 41, no. 5, pp. 576-580.
Kaufman, B. M. ; Summers, P. L. ; Dubois, D. R. ; Cohen, W. H. ; Gentry, M. K. ; Timchak, R. L. ; Burke, D. S. ; Eckels, K. H. / Monoclonal antibodies for dengue virus prM glycoprotein protect mice against lethal dengue infection. In: American Journal of Tropical Medicine and Hygiene. 1989 ; Vol. 41, No. 5. pp. 576-580.
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