TY - JOUR
T1 - Monoclonal antibodies against human pancreatic adenocarcinoma
T2 - Distribution of DU-PAN-2 antigen on glandular epithelia and adenocarcinomas
AU - Borowitz, Michael J.
AU - Tuck, Francis L.
AU - Sindelar, William F.
AU - Fernsten, Philip D.
AU - Metzgar, Richard S.
N1 - Funding Information:
I Received July 22, 1983; accepled December 9, 1983. 2 Supponed by grant IM-326 from the American Cancer Society and by Public Health Service grant CA-32672 from the National Cancer Institule, 3 Depanment of Palhology, Duke UniversilY Medical Center, Durham, N.C. 27710. 4 Departmenl of Microbiology and Immunology, Duke UniversilY Medical Center. S Surgery Branch, Division of Cancer Treatment, National Cancer Inslitute, National Instilutes of Health, Public Health Service, U.S. Department of Health and Human Services, Bethesda, Md. 20205. 6 Address reprint requests to Dr. Melzgar, P.O. Box 3839, Duke University Medical Center, Durham, N.C. 27710. 7 We gralefully acknowledge lhe technical assistance of Nancy Glover, lhe pholographic work of Susan Reeves, and lhe lyping of Nancy Cheek. We are also graleful to Dr. D. Piselsky for performing lhe quantilalive IgM delerminalions.
PY - 1984/5
Y1 - 1984/5
N2 - This report describes the distribution of antigen DU-PAN-2, defined by a monoclonal antibody raised against human pancreatic carcinoma cells, on a variety of tumors and nonneoplastic tissues. With the use of an immunoperoxidase technique, the antigen was detected on 16 of 16 pancreatic carcinomas, on 5 of 5 gallbladder or bile duct carcinomas, on the great majority of stomach adenocarcinomas, and, less commonly, on adenocarcinomas of other primary sites. Substantial intra-tumor heterogeneity of antigen expression was noted. DU-PAN-2 antigen was present on many types of normal glandular epithelial cells but often was more weakly expressed than on the corresponding tumors. The immunomorphology of staining, coupled with biochemical information known about the antigen, supports the notion that the DU-PAN-2 antigen is a mucin-like substance. Its relative restriction of expression on different types of glandular epithelium suggests that DU-PAN-2 antibody might be a useful reagent for helping to determine the site of origin of adenocarcinomas.
AB - This report describes the distribution of antigen DU-PAN-2, defined by a monoclonal antibody raised against human pancreatic carcinoma cells, on a variety of tumors and nonneoplastic tissues. With the use of an immunoperoxidase technique, the antigen was detected on 16 of 16 pancreatic carcinomas, on 5 of 5 gallbladder or bile duct carcinomas, on the great majority of stomach adenocarcinomas, and, less commonly, on adenocarcinomas of other primary sites. Substantial intra-tumor heterogeneity of antigen expression was noted. DU-PAN-2 antigen was present on many types of normal glandular epithelial cells but often was more weakly expressed than on the corresponding tumors. The immunomorphology of staining, coupled with biochemical information known about the antigen, supports the notion that the DU-PAN-2 antigen is a mucin-like substance. Its relative restriction of expression on different types of glandular epithelium suggests that DU-PAN-2 antibody might be a useful reagent for helping to determine the site of origin of adenocarcinomas.
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U2 - 10.1093/jnci/72.5.999
DO - 10.1093/jnci/72.5.999
M3 - Article
C2 - 6371347
AN - SCOPUS:0021277517
SN - 0027-8874
VL - 72
SP - 999
EP - 1005
JO - Journal of the National Cancer Institute
JF - Journal of the National Cancer Institute
IS - 5
ER -