Monoclonal anti-human prostatic acid phosphatase antibodies

Hyun Soon Lillehoj, Byung Kil Choe, Noel R. Rose

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


Hybrid cell lines producing monoclonal antibodies against human prostatic acid phosphatase (E. C. were prepared by the fusion of mouse myeloma cells with the spleen cells of BALB/c mice and Lewis rats immunized with prostatic acid phosphatase (PAP). Approximately 14% of the hybrid cell microcultures which produced specific antibodies were cloned, and 6 eventually yielded stable cell lines. The monoclonal antibodies produced by these 6 hybridomas were characterized for their isotypes, isoelectric points, concentrations and affinities. The specificity of these monoclonal antibodies was further investigated by radioimmunoassay and immunohistochemical methods. All of the 6 monoclonal antibodies exhibited strict specificity for prostatic acid phosphatase.

Original languageEnglish (US)
Pages (from-to)1199-1202
Number of pages4
JournalMolecular Immunology
Issue number9
StatePublished - Sep 1982
Externally publishedYes

ASJC Scopus subject areas

  • Immunology
  • Molecular Biology


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