Abstract
Hybrid cell lines producing monoclonal antibodies against human prostatic acid phosphatase (E. C. 3.1.3.2) were prepared by the fusion of mouse myeloma cells with the spleen cells of BALB/c mice and Lewis rats immunized with prostatic acid phosphatase (PAP). Approximately 14% of the hybrid cell microcultures which produced specific antibodies were cloned, and 6 eventually yielded stable cell lines. The monoclonal antibodies produced by these 6 hybridomas were characterized for their isotypes, isoelectric points, concentrations and affinities. The specificity of these monoclonal antibodies was further investigated by radioimmunoassay and immunohistochemical methods. All of the 6 monoclonal antibodies exhibited strict specificity for prostatic acid phosphatase.
Original language | English (US) |
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Pages (from-to) | 1199-1202 |
Number of pages | 4 |
Journal | Molecular Immunology |
Volume | 19 |
Issue number | 9 |
DOIs | |
State | Published - Sep 1982 |
Externally published | Yes |
ASJC Scopus subject areas
- Immunology
- Molecular Biology