Monoclonal anti-gametocyte antibodies identify an antigen present in all blood stages of Plasmodium falciparum

Aoi Masuda, Fidel Zavala, Victor Nussenzweig, Ruth S. Nussenzweig

Research output: Contribution to journalArticlepeer-review


Two polypeptides of 150 and 130 kDa present in all asexual and sexual blood stages of Plasmodium falciparum have been identified with anti-gametocyte monoclonal antibodies. The apparent molecular mass of these antigens is identical in different developmental stages of the parasite and in different isolates. These antigens are released in the culture supernatant during the process of schizogony and are also detected in the sera of patients undergoing a primary P. falciparum infection. Antibodies against these antigens occur in sera of a large percentage of children and most adults living in malaria-endemic areas, suggesting that they are highly immunogenic. The antigametocyte monoclonal antibodies react with a synthetic peptide (Glu-Glu-Asn-Val)4, present in antigen Pf155 [Perlmann, H. et al. (1984) J. Exp. Med. 159, 1686-1704] and in the ring-infected erythrocyte surface antigen [Coppel, R.L. et al. (1984) Nature 310, 789-792], indicating that these polypeptides are closely related. In contrast, two glycophorin-binding proteins of similar molecular mass [Perkins, M.E. (1984) J. Exp. Med. 160, 788-798] appear to be entirely distinct from the presently described antigens. We failed to observe any in vitro inhibitory activity of the monoclonal antibodies on merozoite invasion and on gametocyte infectivity.

Original languageEnglish (US)
Pages (from-to)213-222
Number of pages10
JournalMolecular and Biochemical Parasitology
Issue number3
StatePublished - Jun 1986
Externally publishedYes


  • Monoclonal anti-gametocyte antibodies
  • Plasmodium falciparum
  • Ring-infected erythrocyte surface antigen
  • Synthetic peptide

ASJC Scopus subject areas

  • Parasitology
  • Molecular Biology


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