Abstract
Understanding of the trafficking of antigen-specific CD8+ T cells in vivo will provide insight about how our immune system controls infectious diseases and cancers. In the current study we used a luciferase-expressing human papillomavirus type 16 (HPV-16) E7-specific CD8 + T cell for adoptive transfer to control E7-expressing TC-1 tumor cells. We used noninvasive luminescence imaging to monitor the trafficking of E7-specific CD8+ T cells over time. We also boosted the luciferase-expressing E7-specific CD8+ T cells in vivo, using E7-expressing vaccinia. We found that injected E7-specific T cells preferentially migrated to the E7-expressing tumor site but not to the E7-negative control tumor site, and increased in number at the tumor site over time. In addition, vaccination with E7-expressing vaccinia led to a significant increase in the number of E7-specific CD8+ T cells at the tumor site, resulting in a significant antitumor effect compared with vaccination with wild-type vaccinia. Thus, our data suggest that the antitumor effects generated by adoptive transfer of E7-specific CD8+ T cells can be significantly enhanced by vaccination with E7-expressing vaccinia and that our system represents a plausible approach to investigate the trafficking and biology of antigen-specific T cells in vivo.
Original language | English (US) |
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Pages (from-to) | 575-588 |
Number of pages | 14 |
Journal | Human gene therapy |
Volume | 18 |
Issue number | 7 |
DOIs | |
State | Published - Jul 2007 |
ASJC Scopus subject areas
- Molecular Medicine
- Molecular Biology
- Genetics