Abstract
Understanding of the trafficking of antigen-specific CD8+ T cells in vivo will provide insight about how our immune system controls infectious diseases and cancers. In the current study we used a luciferase-expressing human papillomavirus type 16 (HPV-16) E7-specific CD8 + T cell for adoptive transfer to control E7-expressing TC-1 tumor cells. We used noninvasive luminescence imaging to monitor the trafficking of E7-specific CD8+ T cells over time. We also boosted the luciferase-expressing E7-specific CD8+ T cells in vivo, using E7-expressing vaccinia. We found that injected E7-specific T cells preferentially migrated to the E7-expressing tumor site but not to the E7-negative control tumor site, and increased in number at the tumor site over time. In addition, vaccination with E7-expressing vaccinia led to a significant increase in the number of E7-specific CD8+ T cells at the tumor site, resulting in a significant antitumor effect compared with vaccination with wild-type vaccinia. Thus, our data suggest that the antitumor effects generated by adoptive transfer of E7-specific CD8+ T cells can be significantly enhanced by vaccination with E7-expressing vaccinia and that our system represents a plausible approach to investigate the trafficking and biology of antigen-specific T cells in vivo.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 575-588 |
| Number of pages | 14 |
| Journal | Human Gene Therapy |
| Volume | 18 |
| Issue number | 7 |
| DOIs | |
| State | Published - Jul 2007 |
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ASJC Scopus subject areas
- Genetics
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Monitoring the trafficking of adoptively transferred antigen-specific CD8-positive T cells in vivo, using noninvasive luminescence imaging. / Kim, Daejin; Hung, Chien-Fu; Wu, Tzyy Choou.
In: Human Gene Therapy, Vol. 18, No. 7, 07.2007, p. 575-588.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Monitoring the trafficking of adoptively transferred antigen-specific CD8-positive T cells in vivo, using noninvasive luminescence imaging
AU - Kim, Daejin
AU - Hung, Chien-Fu
AU - Wu, Tzyy Choou
PY - 2007/7
Y1 - 2007/7
N2 - Understanding of the trafficking of antigen-specific CD8+ T cells in vivo will provide insight about how our immune system controls infectious diseases and cancers. In the current study we used a luciferase-expressing human papillomavirus type 16 (HPV-16) E7-specific CD8 + T cell for adoptive transfer to control E7-expressing TC-1 tumor cells. We used noninvasive luminescence imaging to monitor the trafficking of E7-specific CD8+ T cells over time. We also boosted the luciferase-expressing E7-specific CD8+ T cells in vivo, using E7-expressing vaccinia. We found that injected E7-specific T cells preferentially migrated to the E7-expressing tumor site but not to the E7-negative control tumor site, and increased in number at the tumor site over time. In addition, vaccination with E7-expressing vaccinia led to a significant increase in the number of E7-specific CD8+ T cells at the tumor site, resulting in a significant antitumor effect compared with vaccination with wild-type vaccinia. Thus, our data suggest that the antitumor effects generated by adoptive transfer of E7-specific CD8+ T cells can be significantly enhanced by vaccination with E7-expressing vaccinia and that our system represents a plausible approach to investigate the trafficking and biology of antigen-specific T cells in vivo.
AB - Understanding of the trafficking of antigen-specific CD8+ T cells in vivo will provide insight about how our immune system controls infectious diseases and cancers. In the current study we used a luciferase-expressing human papillomavirus type 16 (HPV-16) E7-specific CD8 + T cell for adoptive transfer to control E7-expressing TC-1 tumor cells. We used noninvasive luminescence imaging to monitor the trafficking of E7-specific CD8+ T cells over time. We also boosted the luciferase-expressing E7-specific CD8+ T cells in vivo, using E7-expressing vaccinia. We found that injected E7-specific T cells preferentially migrated to the E7-expressing tumor site but not to the E7-negative control tumor site, and increased in number at the tumor site over time. In addition, vaccination with E7-expressing vaccinia led to a significant increase in the number of E7-specific CD8+ T cells at the tumor site, resulting in a significant antitumor effect compared with vaccination with wild-type vaccinia. Thus, our data suggest that the antitumor effects generated by adoptive transfer of E7-specific CD8+ T cells can be significantly enhanced by vaccination with E7-expressing vaccinia and that our system represents a plausible approach to investigate the trafficking and biology of antigen-specific T cells in vivo.
UR - http://www.scopus.com/inward/record.url?scp=34547521360&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34547521360&partnerID=8YFLogxK
U2 - 10.1089/hum.2007.038
DO - 10.1089/hum.2007.038
M3 - Article
C2 - 17576157
AN - SCOPUS:34547521360
VL - 18
SP - 575
EP - 588
JO - Human Gene Therapy
JF - Human Gene Therapy
SN - 1043-0342
IS - 7
ER -