Monitoring methylation and gene expression in cancer.

Hetty Carraway, James Herman

Research output: Contribution to journalArticle

Abstract

Identification of patterns of DNA methylation in higher order eukaryotes has become necessary through recognition that disease processes such as cancer can result from abnormal methylation patterns. Abnormal DNA methylation affecting the promoter region of genes can halt their expression, making DNA methylation a marker of gene inactivation. The explosion of studies involving changes in DNA methylation in the last 10 yr, particularly in the study of cancer, are largely the result of two factors: the increasing awareness of the importance of epigenetic silencing in cancer and the improvements in the techniques used to determine changes in DNA methylation. In this chapter, focus is made on the specific methods of how to perform bisulfite modification of DNA and polymerase chain reaction as well as nested methylation specific polymerase chain reaction, and discuss other techniques to evaluate DNA methylation along with the limitations inherent in each process.

Original languageEnglish (US)
Pages (from-to)187-202
Number of pages16
JournalMethods in molecular biology (Clifton, N.J.)
Volume383
StatePublished - 2007

Fingerprint

Gene Expression Profiling
DNA Methylation
Methylation
Neoplasms
Polymerase Chain Reaction
Explosions
Gene Silencing
DNA-Directed DNA Polymerase
Eukaryota
Genetic Promoter Regions
Epigenomics
Genes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Monitoring methylation and gene expression in cancer. / Carraway, Hetty; Herman, James.

In: Methods in molecular biology (Clifton, N.J.), Vol. 383, 2007, p. 187-202.

Research output: Contribution to journalArticle

Carraway, Hetty ; Herman, James. / Monitoring methylation and gene expression in cancer. In: Methods in molecular biology (Clifton, N.J.). 2007 ; Vol. 383. pp. 187-202.
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