Monitoring dynamic changes in lymph metabolome of fasting and fed rats by matrix-assisted laser desorption/ionization-ion mobility mass spectrometry (MALDI-IMMS)

Kimberly Kaplan, Shelley Jackson, Prabha Dwivedi, W. Sean Davidson, Qing Yang, Patrick Tso, William Siems, Amina Woods, Herbert H. Hill

Research output: Contribution to journalArticle

Abstract

Matrix-assisted laser/desorption ionization (MALDI) time-of-flight mass spectrometry (TOF) has been investigated for use in the field of metabolomics; however, difficulties, mainly due to chemical interferences, are typically encountered. By coupling MALDI with ion mobility time-of-flight mass spectrometry (IMMS), isomers and isobars are resolved in mobility space reducing the chemical interference from matrix/background ions. MALDI-IMMS offers the advantages of high sensitivity, high throughput and low sample consumption. For this study, MALDI-IMMS was evaluated by monitoring metabolic changes in lymphatic fluid collected from fasting and fed rats. The number of metabolite features detected in the samples ranged between 1200 and 3400 depending on the duration between the feeding time and lymph sample collection. There were 747 metabolite features that were statistically analyzed by principal component analysis (PCA). From the 3-D score plots of PC1, PC2 and PC3 65 % of the original variation of the system was explained and the differences between the samples were demonstrated.

Original languageEnglish (US)
Pages (from-to)177-184
Number of pages8
JournalInternational Journal for Ion Mobility Spectrometry
Volume16
Issue number3
DOIs
StatePublished - 2013
Externally publishedYes

Fingerprint

Ionization
Mass spectrometry
Rats
Desorption
Ions
Lasers
Monitoring
Metabolites
Isomers
Principal component analysis
Throughput
Fluids

Keywords

  • MALDI-IMMS
  • Metabolomics
  • Principal component analysis

ASJC Scopus subject areas

  • Spectroscopy

Cite this

Monitoring dynamic changes in lymph metabolome of fasting and fed rats by matrix-assisted laser desorption/ionization-ion mobility mass spectrometry (MALDI-IMMS). / Kaplan, Kimberly; Jackson, Shelley; Dwivedi, Prabha; Davidson, W. Sean; Yang, Qing; Tso, Patrick; Siems, William; Woods, Amina; Hill, Herbert H.

In: International Journal for Ion Mobility Spectrometry, Vol. 16, No. 3, 2013, p. 177-184.

Research output: Contribution to journalArticle

Kaplan, Kimberly ; Jackson, Shelley ; Dwivedi, Prabha ; Davidson, W. Sean ; Yang, Qing ; Tso, Patrick ; Siems, William ; Woods, Amina ; Hill, Herbert H. / Monitoring dynamic changes in lymph metabolome of fasting and fed rats by matrix-assisted laser desorption/ionization-ion mobility mass spectrometry (MALDI-IMMS). In: International Journal for Ion Mobility Spectrometry. 2013 ; Vol. 16, No. 3. pp. 177-184.
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AU - Yang, Qing

AU - Tso, Patrick

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AU - Woods, Amina

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