Monitoring antigen-specific T cells using MHC-Ig dimers.

J. P. Schneck, J. E. Slansky, S. M. O'Herrin, T. F. Greten

Research output: Contribution to journalArticlepeer-review

Abstract

The lack of high affinity reagents has made distinguishing T cells on the basis of antigen specificity difficult to accomplish. This unit provides protocols that utilize innovations in molecular design to permit construction of soluble multivalent MHC complexes (MHC-Ig dimers) with high avidity for cognate T cell receptors. MHC-Ig dimers display stable binding properties when they interact with antigen-specific T cells thus allowing their use in the staining of antigen-specific T cells by flow cytometry. Methods for constructing and detecting these MHC-Ig dimers are included along with protocols for applying their use for the quantitation of antigen-specific T cells.

Original languageEnglish (US)
Pages (from-to)Unit 17.2
JournalCurrent protocols in immunology / edited by John E. Coligan ... [et al.]
VolumeChapter 17
DOIs
StatePublished - May 2001

ASJC Scopus subject areas

  • Immunology

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