Abstract
The lack of high affinity reagents has made distinguishing T cells on the basis of antigen specificity difficult to accomplish. This unit provides protocols that utilize innovations in molecular design to permit construction of soluble multivalent MHC complexes (MHC-Ig dimers) with high avidity for cognate T cell receptors. MHC-Ig dimers display stable binding properties when they interact with antigen-specific T cells thus allowing their use in the staining of antigen-specific T cells by flow cytometry. Methods for constructing and detecting these MHC-Ig dimers are included along with protocols for applying their use for the quantitation of antigen-specific T cells.
Original language | English (US) |
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Pages (from-to) | Unit 17.2 |
Journal | Current protocols in immunology / edited by John E. Coligan ... [et al.] |
Volume | Chapter 17 |
DOIs | |
State | Published - May 2001 |
ASJC Scopus subject areas
- Immunology