Molecular Testing of Serial Blood Specimens from Patients with Early Lyme Disease during Treatment Reveals Changing Coinfection with Mixtures of Borrelia burgdorferi Genotypes

Michael R. Mosel, Heather E. Carolan, Alison W. Rebman, Steven Castro, Christian Massire, David J. Ecker, Mark J Soloski, John Aucott, Mark W. Eshoo

Research output: Contribution to journalArticle

Abstract

Borrelia burgdorferi is the etiological agent of Lyme disease. In the current study, we used direct-detection PCR and electrospray ionization mass spectrometry to monitor and genotype B. burgdorferi isolates from serially collected whole-blood specimens from patients clinically diagnosed with early Lyme disease before and during 21 days of antibiotic therapy. B. burgdorferi isolates were detected up to 3 weeks after the initiation of antibiotic treatment, with ratios of coinfecting B. burgdorferi genotypes changing over time.

Original languageEnglish (US)
Article numbere00237-19
JournalAntimicrobial agents and chemotherapy
Volume63
Issue number7
DOIs
StatePublished - Jan 1 2019

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Borrelia burgdorferi
Lyme Disease
Coinfection
Genotype
Anti-Bacterial Agents
Electrospray Ionization Mass Spectrometry
Therapeutics
Polymerase Chain Reaction

Keywords

  • Antibiotic
  • Borrelia burgdorferi
  • Genotype
  • Lyme disease
  • Time course

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

Cite this

Molecular Testing of Serial Blood Specimens from Patients with Early Lyme Disease during Treatment Reveals Changing Coinfection with Mixtures of Borrelia burgdorferi Genotypes. / Mosel, Michael R.; Carolan, Heather E.; Rebman, Alison W.; Castro, Steven; Massire, Christian; Ecker, David J.; Soloski, Mark J; Aucott, John; Eshoo, Mark W.

In: Antimicrobial agents and chemotherapy, Vol. 63, No. 7, e00237-19, 01.01.2019.

Research output: Contribution to journalArticle

Mosel, Michael R. ; Carolan, Heather E. ; Rebman, Alison W. ; Castro, Steven ; Massire, Christian ; Ecker, David J. ; Soloski, Mark J ; Aucott, John ; Eshoo, Mark W. / Molecular Testing of Serial Blood Specimens from Patients with Early Lyme Disease during Treatment Reveals Changing Coinfection with Mixtures of Borrelia burgdorferi Genotypes. In: Antimicrobial agents and chemotherapy. 2019 ; Vol. 63, No. 7.
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