Molecular structure of the water channel through aquaporin CHIP: The hourglass model

Jin Sup Jung, Gregory M. Prestont, Barbara L. Smith, William B Guggino, Peter C Agre

Research output: Contribution to journalArticle

Abstract

Aquaporin channel-forming integral protein (CHIP) is the first characterized water channel protein (genome symbol AQP1), but the molecular structure of the aqueous pathway through CHIP remains undefined. The two halves of CHIP are sequence-related, and each has three bilayer-spanning domains with the motif asparagineproline-alanine (NPA) at residues 76-78 (in cytoplasmic loop B) and 192-194 (in extracellular loop E). The NPA motifs are oriented 180° to each other, and the second NPA is near cysteine 189, the known site where mercurials inhibit osmotic water permeability (Pf). When expressed in Xenopus oocytes, the double mutant A73C/ C189S exhibited high, mercurial-sensitive Pf similar to wild-type CHIP. Conservative substitutions of slightly greater mass in or near NPA motifs in loop B or loop E in CHIP caused reduced Pf and failure of the protein to localize at the plasma membrane. Certain nonfunctional loop E mutants complemented the truncation mutant D237Z. Formation of mixed oligomers was demonstrated by velocity sedimentation, immunoprecipitation, and analysis of dimeric-CHIP polypeptides. Cellular distributions of individual mutants or complementing pairs of mutants were verified by plasma membrane isolation and confocal microscopy. An hourglass structural model is proposed in which a cytoplasmic chamber (loop B) connects within the membrane to an extracellular chamber (loop E) forming a single, narrow aqueous pathway through each of the CHIP subunits; subunit oligomerization may provide the vertical symmetry necessary for residence within the lipid bilayer.

Original languageEnglish (US)
Pages (from-to)14648-14654
Number of pages7
JournalJournal of Biological Chemistry
Volume269
Issue number20
StatePublished - May 20 1994

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Aquaporins
Molecular Structure
Molecular structure
Proteins
Cell membranes
Cell Membrane
Oligomerization
Lipid bilayers
Confocal microscopy
Structural Models
Protein Subunits
Lipid Bilayers
Xenopus
Sedimentation
Immunoprecipitation
Oligomers
Confocal Microscopy
Alanine
Oocytes
Cysteine

ASJC Scopus subject areas

  • Biochemistry

Cite this

Molecular structure of the water channel through aquaporin CHIP : The hourglass model. / Jung, Jin Sup; Prestont, Gregory M.; Smith, Barbara L.; Guggino, William B; Agre, Peter C.

In: Journal of Biological Chemistry, Vol. 269, No. 20, 20.05.1994, p. 14648-14654.

Research output: Contribution to journalArticle

Jung, Jin Sup ; Prestont, Gregory M. ; Smith, Barbara L. ; Guggino, William B ; Agre, Peter C. / Molecular structure of the water channel through aquaporin CHIP : The hourglass model. In: Journal of Biological Chemistry. 1994 ; Vol. 269, No. 20. pp. 14648-14654.
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