Molecular specific and sensitive detection of pyrazinamide and its metabolite pyrazinoic acid by means of surface enhanced raman spectroscopy employing in situ prepared colloids

Anna Muehlig; Izabella J. Jahn; Jan Heidler; Martin Jahn; Karina Weber; Patricia Sheen; Mirko Zimic; Dana Cialla-May; Juergen Popp

doi:10.3390/app9122511

Molecular specific and sensitive detection of pyrazinamide and its metabolite pyrazinoic acid by means of surface enhanced raman spectroscopy employing in situ prepared colloids

Anna Muehlig, Izabella J. Jahn, Jan Heidler, Martin Jahn, Karina Weber, Patricia Sheen, Mirko Zimic, Dana Cialla-May, Juergen Popp

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

The prodrug pyrazinamide (PZA) is metabolized by the mycobacteria to pyrazinoic acid (POA), which is expelled into the extracellular environment. PZA resistance is highly associated to a lack of POA efflux. Thus, by detecting a reduction of the concentration of POA in the extracellular environment, by means of lab-on-a-chip (LoC)-SERS (surface-enhanced Raman spectroscopy), an alternative approach for the discrimination of PZA resistant mycobacteria is introduced. A droplet-based microfluidic SERS device has been employed to illustrate the potential of the LoC-SERS method for the discrimination of PZA resistant mycobacteria. The two analytes were detected discretely in aqueous solution with a limit of detection of 27 μm for PZA and 21 μm for POA. The simultaneous detection of PZA and POA in aqueous mixtures could be realized within a concentration range from 20 μm to 50 μm for PZA and from 50 μm to 80 μm for POA.

Original language	English (US)
Article number	511
Journal	Applied Sciences (Switzerland)
Volume	9
Issue number	12
DOIs	https://doi.org/10.3390/app9122511
State	Published - Jun 1 2019
Externally published	Yes

Keywords

Drug resistant mycobacteria
Lab-on-a-Chip (LoC)
Personal medicine
Quantitative SERS detection of PZA and POA
Surface-enhanced Raman spectroscopy (SERS)

ASJC Scopus subject areas

General Materials Science
Instrumentation
General Engineering
Process Chemistry and Technology
Computer Science Applications
Fluid Flow and Transfer Processes

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10.3390/app9122511

Cite this

Muehlig, A., Jahn, I. J., Heidler, J., Jahn, M., Weber, K., Sheen, P., Zimic, M., Cialla-May, D., & Popp, J. (2019). Molecular specific and sensitive detection of pyrazinamide and its metabolite pyrazinoic acid by means of surface enhanced raman spectroscopy employing in situ prepared colloids. Applied Sciences (Switzerland), 9(12), Article 511. https://doi.org/10.3390/app9122511

Molecular specific and sensitive detection of pyrazinamide and its metabolite pyrazinoic acid by means of surface enhanced raman spectroscopy employing in situ prepared colloids. / Muehlig, Anna; Jahn, Izabella J.; Heidler, Jan et al.
In: Applied Sciences (Switzerland), Vol. 9, No. 12, 511, 01.06.2019.

Research output: Contribution to journal › Article › peer-review

Muehlig, A, Jahn, IJ, Heidler, J, Jahn, M, Weber, K, Sheen, P, Zimic, M, Cialla-May, D & Popp, J 2019, 'Molecular specific and sensitive detection of pyrazinamide and its metabolite pyrazinoic acid by means of surface enhanced raman spectroscopy employing in situ prepared colloids', Applied Sciences (Switzerland), vol. 9, no. 12, 511. https://doi.org/10.3390/app9122511

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title = "Molecular specific and sensitive detection of pyrazinamide and its metabolite pyrazinoic acid by means of surface enhanced raman spectroscopy employing in situ prepared colloids",

abstract = "The prodrug pyrazinamide (PZA) is metabolized by the mycobacteria to pyrazinoic acid (POA), which is expelled into the extracellular environment. PZA resistance is highly associated to a lack of POA efflux. Thus, by detecting a reduction of the concentration of POA in the extracellular environment, by means of lab-on-a-chip (LoC)-SERS (surface-enhanced Raman spectroscopy), an alternative approach for the discrimination of PZA resistant mycobacteria is introduced. A droplet-based microfluidic SERS device has been employed to illustrate the potential of the LoC-SERS method for the discrimination of PZA resistant mycobacteria. The two analytes were detected discretely in aqueous solution with a limit of detection of 27 μm for PZA and 21 μm for POA. The simultaneous detection of PZA and POA in aqueous mixtures could be realized within a concentration range from 20 μm to 50 μm for PZA and from 50 μm to 80 μm for POA.",

keywords = "Drug resistant mycobacteria, Lab-on-a-Chip (LoC), Personal medicine, Quantitative SERS detection of PZA and POA, Surface-enhanced Raman spectroscopy (SERS)",

author = "Anna Muehlig and Jahn, {Izabella J.} and Jan Heidler and Martin Jahn and Karina Weber and Patricia Sheen and Mirko Zimic and Dana Cialla-May and Juergen Popp",

note = "Funding Information: Funding: We gratefully acknowledge the Federal Ministry of Education and Research, Germany (BMBF) for supporting the InfectoGnostics (13GW0096F) and EXASENS (13N13856) project grants as well as BMBF and German Aerospace Center (DLR) for supporting the Myco-NET2 (01DN15028) project grant. We thank for funding the projects ERANET LAC ELAC2014/HID-0352, CONCYTEC-FONDECYT-Peru 086-2015, and the Wellcome Trust Intermediate Fellowship (grant 099805/Z/12/Z). Publisher Copyright: {\textcopyright} 2019 by the authors.",

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T1 - Molecular specific and sensitive detection of pyrazinamide and its metabolite pyrazinoic acid by means of surface enhanced raman spectroscopy employing in situ prepared colloids

AU - Muehlig, Anna

AU - Jahn, Izabella J.

AU - Heidler, Jan

AU - Jahn, Martin

AU - Weber, Karina

AU - Sheen, Patricia

AU - Zimic, Mirko

AU - Cialla-May, Dana

AU - Popp, Juergen

N1 - Funding Information: Funding: We gratefully acknowledge the Federal Ministry of Education and Research, Germany (BMBF) for supporting the InfectoGnostics (13GW0096F) and EXASENS (13N13856) project grants as well as BMBF and German Aerospace Center (DLR) for supporting the Myco-NET2 (01DN15028) project grant. We thank for funding the projects ERANET LAC ELAC2014/HID-0352, CONCYTEC-FONDECYT-Peru 086-2015, and the Wellcome Trust Intermediate Fellowship (grant 099805/Z/12/Z). Publisher Copyright: © 2019 by the authors.

PY - 2019/6/1

Y1 - 2019/6/1

N2 - The prodrug pyrazinamide (PZA) is metabolized by the mycobacteria to pyrazinoic acid (POA), which is expelled into the extracellular environment. PZA resistance is highly associated to a lack of POA efflux. Thus, by detecting a reduction of the concentration of POA in the extracellular environment, by means of lab-on-a-chip (LoC)-SERS (surface-enhanced Raman spectroscopy), an alternative approach for the discrimination of PZA resistant mycobacteria is introduced. A droplet-based microfluidic SERS device has been employed to illustrate the potential of the LoC-SERS method for the discrimination of PZA resistant mycobacteria. The two analytes were detected discretely in aqueous solution with a limit of detection of 27 μm for PZA and 21 μm for POA. The simultaneous detection of PZA and POA in aqueous mixtures could be realized within a concentration range from 20 μm to 50 μm for PZA and from 50 μm to 80 μm for POA.

AB - The prodrug pyrazinamide (PZA) is metabolized by the mycobacteria to pyrazinoic acid (POA), which is expelled into the extracellular environment. PZA resistance is highly associated to a lack of POA efflux. Thus, by detecting a reduction of the concentration of POA in the extracellular environment, by means of lab-on-a-chip (LoC)-SERS (surface-enhanced Raman spectroscopy), an alternative approach for the discrimination of PZA resistant mycobacteria is introduced. A droplet-based microfluidic SERS device has been employed to illustrate the potential of the LoC-SERS method for the discrimination of PZA resistant mycobacteria. The two analytes were detected discretely in aqueous solution with a limit of detection of 27 μm for PZA and 21 μm for POA. The simultaneous detection of PZA and POA in aqueous mixtures could be realized within a concentration range from 20 μm to 50 μm for PZA and from 50 μm to 80 μm for POA.

KW - Drug resistant mycobacteria

KW - Lab-on-a-Chip (LoC)

KW - Personal medicine

KW - Quantitative SERS detection of PZA and POA

KW - Surface-enhanced Raman spectroscopy (SERS)

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ER -

Molecular specific and sensitive detection of pyrazinamide and its metabolite pyrazinoic acid by means of surface enhanced raman spectroscopy employing in situ prepared colloids

Abstract

Keywords

ASJC Scopus subject areas

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