Molecular screening for Neisseria gonorrhoeae antimicrobial resistance markers in Nigerian men who have sex with men and transgender women

Justin Hardick, Trevor A. Crowell, Kara Lombardi, Akindiran Akintunde, Sunday Odeyemi, Andrew Ivo, George Eluwa, Jean Njab, Stefan Baral, Rebecca G. Nowak, Thomas C Quinn, Kent Barbian, Sarah Anzick, Sylvia Adebajo, Manhattan E. Charurat, Julie Ake, Charlotte A Gaydos

Research output: Contribution to journalArticle

Abstract

Antimicrobial-resistant Neisseria gonorrhoeae (NG) is a global public health issue that threatens effectiveness of current treatments of NG. Increased use of nucleic acid amplification tests (NAATs) in lieu of cultures makes obtaining clinical isolates for susceptibility testing difficult and samples collected in commercial transport buffer for NAATs do not preserve viable organism, while molecular methods of assessing antibiotic susceptibility do not require viable organism. We evaluated 243 NG-positive samples in Aptima transport media including urine, oral, and rectal swabs from Nigerian men who have sex with men for markers to penicillinase-producing NG, ciprofloxacin (GyrA and ParC mutations), and extended spectrum cephalosporins (ESCs, PenA mosaic [allele X], PonA, mtrR, PorB mutations) by real-time PCR. NG DNA was recovered in 75% (183/243) of samples. Of these, 93% (171/183) were positive for at least one resistance marker. We observed a prevalence of dual resistance markers to penicillin and ciprofloxacin at 46.2% (79/171). Six percent of samples (10/171) tested positive for the PenA mosaic (allele X) ESC marker. These data indicate that antibiotic-resistant NG is common in Nigeria. Laboratory and clinical capacity building in Nigeria should include development of methods to culture NG and determine antimicrobial susceptibility.

Original languageEnglish (US)
JournalInternational Journal of STD and AIDS
DOIs
StateAccepted/In press - Jan 1 2018

Fingerprint

Transgender Persons
Neisseria gonorrhoeae
Nucleic Acid Amplification Techniques
Ciprofloxacin
Nigeria
Alleles
Anti-Bacterial Agents
Capacity Building
Penicillinase
Mutation
Cephalosporins
Penicillins
Real-Time Polymerase Chain Reaction
Buffers
Public Health
Urine
DNA

Keywords

  • Africa
  • antibiotic
  • gonorrhea

ASJC Scopus subject areas

  • Dermatology
  • Public Health, Environmental and Occupational Health
  • Pharmacology (medical)
  • Infectious Diseases

Cite this

Molecular screening for Neisseria gonorrhoeae antimicrobial resistance markers in Nigerian men who have sex with men and transgender women. / Hardick, Justin; Crowell, Trevor A.; Lombardi, Kara; Akintunde, Akindiran; Odeyemi, Sunday; Ivo, Andrew; Eluwa, George; Njab, Jean; Baral, Stefan; Nowak, Rebecca G.; Quinn, Thomas C; Barbian, Kent; Anzick, Sarah; Adebajo, Sylvia; Charurat, Manhattan E.; Ake, Julie; Gaydos, Charlotte A.

In: International Journal of STD and AIDS, 01.01.2018.

Research output: Contribution to journalArticle

Hardick, J, Crowell, TA, Lombardi, K, Akintunde, A, Odeyemi, S, Ivo, A, Eluwa, G, Njab, J, Baral, S, Nowak, RG, Quinn, TC, Barbian, K, Anzick, S, Adebajo, S, Charurat, ME, Ake, J & Gaydos, CA 2018, 'Molecular screening for Neisseria gonorrhoeae antimicrobial resistance markers in Nigerian men who have sex with men and transgender women', International Journal of STD and AIDS. https://doi.org/10.1177/0956462418780050
Hardick, Justin ; Crowell, Trevor A. ; Lombardi, Kara ; Akintunde, Akindiran ; Odeyemi, Sunday ; Ivo, Andrew ; Eluwa, George ; Njab, Jean ; Baral, Stefan ; Nowak, Rebecca G. ; Quinn, Thomas C ; Barbian, Kent ; Anzick, Sarah ; Adebajo, Sylvia ; Charurat, Manhattan E. ; Ake, Julie ; Gaydos, Charlotte A. / Molecular screening for Neisseria gonorrhoeae antimicrobial resistance markers in Nigerian men who have sex with men and transgender women. In: International Journal of STD and AIDS. 2018.
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