Molecular markers of radiation induced attenuation in intrahepatic plasmodium falciparum parasites

Miranda S. Oakley, Nitin Verma, Hong Zheng, Vivek Anantharaman, Kazuyo Takeda, Yamei Gao, Timothy G. Myers, Phuong Thao Pham, Babita Mahajan, Nirbhay Kumar, Davison Sangweme, Abhai Tripathi, Godfree Mlambo, L. Aravind, Sanjai Kumar

Research output: Contribution to journalArticle

Abstract

Experimental immunization with radiation attenuated sporozoites (RAS) and genetically attenuated sporozoites has proved to be a promising approach for malaria vaccine development. However, parasite biomarkers of growth attenuation and enhanced immune protection in response to radiation remain poorly understood. Here, we report on the effect of an attenuating dose of ã-irradiation (15 krad) on the Plasmodium falciparum sporozoite (PfSPZ) ultrastructure by electron microscopy, growth rate of liver stage P. falciparum in liver cell cultures, and genome-wide transcriptional profile of liver stage parasites by microarray. We find that ã-irradiation treated PfSPZ retained a normal cellular structure except that they were vacuous with a partially disrupted plasma membrane and inner membrane complex. A similar infection rate was observed by ã-irradiation-treated and untreated PfSPZ in human HCO-4 liver cells (0.47% versus 0.49%, respectively) on day 3 post-infection. In the microarray studies, cumulatively, 180 liver stage parasite genes were significantly transcriptionally altered on day 3 and/or 6 post-infection. Among the transcriptionally altered biomarkers, we identified a signature of seven candidate parasite genes that associated with functionally diverse pathways that may regulate radiation induced cell cycle arrest of the parasite within the hepatocyte. A repertoire of 14 genes associated with protein translation is transcriptionally overexpressed within the parasite by radiation. Additionally, 37 genes encode proteins expressed on the cell surface or exported into the host cell, 4 encode membrane associated transporters, and 10 encode proteins related to misfolding and stress-related protein processing. These results have significantly increased the repertoire of novel targets for 1) biomarkers of safety to define proper attenuation, 2) generating genetically attenuated parasite vaccine candidates, and 3) subunit candidate vaccines against liver stage malaria.

Original languageEnglish (US)
Article numbere0166814
JournalPLoS One
Volume11
Issue number12
DOIs
StatePublished - Dec 1 2016

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Plasmodium falciparum
Sporozoites
sporozoites
Parasites
Liver
Radiation
parasites
genetic markers
Genes
Biomarkers
hepatocytes
biomarkers
liver
irradiation
Irradiation
Microarrays
Proteins
Vaccines
genes
proteins

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Oakley, M. S., Verma, N., Zheng, H., Anantharaman, V., Takeda, K., Gao, Y., ... Kumar, S. (2016). Molecular markers of radiation induced attenuation in intrahepatic plasmodium falciparum parasites. PLoS One, 11(12), [e0166814]. https://doi.org/10.1371/journal.pone.0166814

Molecular markers of radiation induced attenuation in intrahepatic plasmodium falciparum parasites. / Oakley, Miranda S.; Verma, Nitin; Zheng, Hong; Anantharaman, Vivek; Takeda, Kazuyo; Gao, Yamei; Myers, Timothy G.; Pham, Phuong Thao; Mahajan, Babita; Kumar, Nirbhay; Sangweme, Davison; Tripathi, Abhai; Mlambo, Godfree; Aravind, L.; Kumar, Sanjai.

In: PLoS One, Vol. 11, No. 12, e0166814, 01.12.2016.

Research output: Contribution to journalArticle

Oakley, MS, Verma, N, Zheng, H, Anantharaman, V, Takeda, K, Gao, Y, Myers, TG, Pham, PT, Mahajan, B, Kumar, N, Sangweme, D, Tripathi, A, Mlambo, G, Aravind, L & Kumar, S 2016, 'Molecular markers of radiation induced attenuation in intrahepatic plasmodium falciparum parasites', PLoS One, vol. 11, no. 12, e0166814. https://doi.org/10.1371/journal.pone.0166814
Oakley MS, Verma N, Zheng H, Anantharaman V, Takeda K, Gao Y et al. Molecular markers of radiation induced attenuation in intrahepatic plasmodium falciparum parasites. PLoS One. 2016 Dec 1;11(12). e0166814. https://doi.org/10.1371/journal.pone.0166814
Oakley, Miranda S. ; Verma, Nitin ; Zheng, Hong ; Anantharaman, Vivek ; Takeda, Kazuyo ; Gao, Yamei ; Myers, Timothy G. ; Pham, Phuong Thao ; Mahajan, Babita ; Kumar, Nirbhay ; Sangweme, Davison ; Tripathi, Abhai ; Mlambo, Godfree ; Aravind, L. ; Kumar, Sanjai. / Molecular markers of radiation induced attenuation in intrahepatic plasmodium falciparum parasites. In: PLoS One. 2016 ; Vol. 11, No. 12.
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abstract = "Experimental immunization with radiation attenuated sporozoites (RAS) and genetically attenuated sporozoites has proved to be a promising approach for malaria vaccine development. However, parasite biomarkers of growth attenuation and enhanced immune protection in response to radiation remain poorly understood. Here, we report on the effect of an attenuating dose of {\~a}-irradiation (15 krad) on the Plasmodium falciparum sporozoite (PfSPZ) ultrastructure by electron microscopy, growth rate of liver stage P. falciparum in liver cell cultures, and genome-wide transcriptional profile of liver stage parasites by microarray. We find that {\~a}-irradiation treated PfSPZ retained a normal cellular structure except that they were vacuous with a partially disrupted plasma membrane and inner membrane complex. A similar infection rate was observed by {\~a}-irradiation-treated and untreated PfSPZ in human HCO-4 liver cells (0.47{\%} versus 0.49{\%}, respectively) on day 3 post-infection. In the microarray studies, cumulatively, 180 liver stage parasite genes were significantly transcriptionally altered on day 3 and/or 6 post-infection. Among the transcriptionally altered biomarkers, we identified a signature of seven candidate parasite genes that associated with functionally diverse pathways that may regulate radiation induced cell cycle arrest of the parasite within the hepatocyte. A repertoire of 14 genes associated with protein translation is transcriptionally overexpressed within the parasite by radiation. Additionally, 37 genes encode proteins expressed on the cell surface or exported into the host cell, 4 encode membrane associated transporters, and 10 encode proteins related to misfolding and stress-related protein processing. These results have significantly increased the repertoire of novel targets for 1) biomarkers of safety to define proper attenuation, 2) generating genetically attenuated parasite vaccine candidates, and 3) subunit candidate vaccines against liver stage malaria.",
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