Molecular level studies on binding modes of labeling molecules with polyalanine peptides

Xiaobo Mao, Chenxuan Wang, Xiaojing Ma, Min Zhang, Lei Liu, Lan Zhang, Lin Niu, Qindao Zeng, Yanlian Yang, Chen Wang

Research output: Contribution to journalArticle


In this work, the binding modes of typical labeling molecules (thioflavin T (ThT), Congo red (CR) and copper(ii) phthalocyanine tetrasulfonic acid tetrasodium salt (PcCu(SO3Na)4)) on pentaalanine, which is a model peptide segment of amyloid peptides, have been resolved at the molecular level by using scanning tunneling microscopy (STM). In the STM images, ThT molecules are predominantly adsorbed parallel to the peptide strands and two binding modes could be identified. It was found that ThT molecules are preferentially binding on top of the peptide strand, and the mode of intercalated between neighboring peptides also exists. The parallel binding mode of CR molecules can be observed with pentaalanine peptides. Besides the binding modes of labeling molecules, the CR and PcCu(SO3Na)4 display different adsorption affinity with the pentaalanine peptides. The results could be beneficial for obtaining molecular level insight of the interactions between labeling molecules and peptides.

Original languageEnglish (US)
Pages (from-to)1592-1599
Number of pages8
Issue number4
Publication statusPublished - Apr 2011
Externally publishedYes


ASJC Scopus subject areas

  • Materials Science(all)

Cite this

Mao, X., Wang, C., Ma, X., Zhang, M., Liu, L., Zhang, L., ... Wang, C. (2011). Molecular level studies on binding modes of labeling molecules with polyalanine peptides. Nanoscale, 3(4), 1592-1599.