Molecular-level evidence of the surface-induced transformation of peptide structures revealed by scanning tunneling microscopy

Xiaobo Mao, Yibing Wang, Lei Liu, Lin Niu, Yanlian Yang, Chen Wang

Research output: Contribution to journalArticle

Abstract

Molecular structures of polypeptide molecules (DELERRIRELEARIK) adsorbed at liquid - solid interfaces of graphite have been studied using scanning tunneling microscopy (STM). The polypeptide is originally stable with an a-helical conformation in solution and in its crystal states. STM observations reveal that the adsorbed polypeptides form homogeneous β-sheet-like assemblies on the graphite surface. The separation (4.7 ± 0.1 Å) between two neighboring polypeptides and the full lengths of the polypeptides determined from STM images suggest distinctively different molecular conformations from the a-helical structure. The N 1s peak in the X-ray photoelectron spectroscopy (XPS) spectrum confirmed the presence of polypeptides on the graphite surface. In addition, the circular dichroism (CD) results provide supporting evidence that the polypeptides would undergo a structural transformation to β-sheet secondary structure upon the addition of graphite particles to the peptide solution. Such conformational rearrangements upon adsorption on a hydrophobic surface could benefit the studies on protein - surface interactions.

Original languageEnglish (US)
Pages (from-to)8849-8853
Number of pages5
JournalLangmuir
Volume25
Issue number16
DOIs
StatePublished - Aug 18 2009
Externally publishedYes

Fingerprint

Polypeptides
polypeptides
Scanning tunneling microscopy
Peptides
peptides
scanning tunneling microscopy
Graphite
graphite
Conformations
Dichroism
liquid-solid interfaces
surface reactions
assemblies
Molecular structure
dichroism
molecular structure
X ray photoelectron spectroscopy
photoelectron spectroscopy
Membrane Proteins
proteins

ASJC Scopus subject areas

  • Electrochemistry
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Materials Science(all)
  • Spectroscopy

Cite this

Molecular-level evidence of the surface-induced transformation of peptide structures revealed by scanning tunneling microscopy. / Mao, Xiaobo; Wang, Yibing; Liu, Lei; Niu, Lin; Yang, Yanlian; Wang, Chen.

In: Langmuir, Vol. 25, No. 16, 18.08.2009, p. 8849-8853.

Research output: Contribution to journalArticle

Mao, Xiaobo ; Wang, Yibing ; Liu, Lei ; Niu, Lin ; Yang, Yanlian ; Wang, Chen. / Molecular-level evidence of the surface-induced transformation of peptide structures revealed by scanning tunneling microscopy. In: Langmuir. 2009 ; Vol. 25, No. 16. pp. 8849-8853.
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