Molecular interactions of complement receptors on B lymphocytes: A CR1/CR2 complex distinct from the CR2/CD19 complex

David A. Tuveson, Joseph M. Ahearn, Alan K. Matsumoto, Douglas T. Fearon

Research output: Contribution to journalArticle

Abstract

The complement system augments the humoral immune response to low concentrations of antigen. This effect may be partly mediated by complement receptors on the surface of B lymphocytes that bind immunogenic complexes bearing fragments of C3 and C4. We have shown by immunoprecipitation analysis that the two complement receptors expressed by B lymphocytes, complement receptor 1 (CR1) and CR2, form a detergent-sensitive complex on the surface of tonsillar B lymphocytes and on K562 erythroleukemia cells that were co-transfected with cDNAs encoding CR1 and CR2. The CR1/CR2 complex is distinct from the CR2/CD19 complex and may assist B cell activation by efficiently capturing C3b-containing immunogens and maintaining such immunogens on the B cell after CR1 and factor I-mediated cleavage to iC3b and C3dg. The complement activating immunogen may then trigger signal transduction by the CR1/CR2 complex, the CR2/CD19 complex, or membrane immunoglobulin.

Original languageEnglish (US)
Pages (from-to)1083-1089
Number of pages7
JournalJournal of Experimental Medicine
Volume173
Issue number5
StatePublished - May 1 1991

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Complement C1
Complement Receptors
B-Lymphocytes
Complement C3b
Leukemia, Erythroblastic, Acute
K562 Cells
Humoral Immunity
Immunoprecipitation
Detergents
Immunoglobulins
Signal Transduction
Complementary DNA
Antigens
Membranes

ASJC Scopus subject areas

  • Immunology

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Molecular interactions of complement receptors on B lymphocytes : A CR1/CR2 complex distinct from the CR2/CD19 complex. / Tuveson, David A.; Ahearn, Joseph M.; Matsumoto, Alan K.; Fearon, Douglas T.

In: Journal of Experimental Medicine, Vol. 173, No. 5, 01.05.1991, p. 1083-1089.

Research output: Contribution to journalArticle

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